摘要
目的:探讨在浸润、分化程度、组织学类型和转移中,癌组织细胞外基质(LN和FN)的含量改变和分布规律.方法:采用S-P免疫组化染色法观察了257例癌组织的LN和FN的含量分布.染色程度分为(-)、(+)和(++),将癌按浸润程度,分化程度、组织学类型和有否转移进行分组.结果:基底膜LN和FN在非浸润癌时连续完整,微浸润区可见分段或缺失,浸润癌时含量明显减少,分布不规则.两者在分化好组和分化差组的表达具有显著性差异(P<0.01).在已分化的各组织学类型之间,LN和FN的表达无明显差异(P<0.05),在转移和未转移组的表达也无明显差异(P>0.05).97%以上的癌间质中存在FN阳性物.结论:基底膜LN和FN是否完整对于鉴别癌是否有浸润有重要的参考价值,其含量随肿瘤细胞分化程度的降低而减少,而与组织学类型和转移无明显关系.间质FN的存在和分布与肿瘤的分化程度、组织学类型及淋巴结转移均无明显关系,可能反映机体对肿瘤的防御和抵抗功能的状态.
Objective:Exploring the change of amount and distribution of laminin(LN) and fibronectin(FN) in invasion, dif-ferentiation, histological types and metastasis of carcinoma. Methods: The distribution of LN and FN in 257 cases with carcinoma was examined with S-P immunohistochemical procedure. Staining grade was divided to ( - ), ( + )and (++) . The groups were divided according to the degree of invasion, differentiation, histological types and the presence of metastasis of tumors. Results: LN and FN in basement membrane(BM) were continuous and intact in noninvasive carcinoma, but local disruptions were found in microinvasive ar-eas. In invasive carcinoma, the amount of LN and FN decreased with irregular distribution or was absent. For the expression of LN and FN, there was a significant difference between well - differentiation and poorly - dirrerentiation groups (P < 0.01), but no obvi-ous difference neither among the histological type of differentiated carcinoma nor between metastasis group and non ?metastasis group (P>0.05). Depositions of stromal FN were found in most tumors( >97% ). Conclusion: Intact BM of LN and FN has an impor-tant value in distinguishing tumor invasion. The amount of LN and FN in BM decrease with the lowering degree of differentiation of the tumors, but has no correlation with the histological types and metastasis. The presence of stromal FN fails to correlated with the tumor differentiation, histological type and metastasis, but may reflect defensive and resistant function of body.
出处
《临床肿瘤学杂志》
CAS
1999年第1期3-6,共4页
Chinese Clinical Oncology
基金
黑龙江省自然科学基金资助项目(D94019)
