摘要
目的 :研究高糖对人肾小球系膜细胞丝裂原蛋白激酶 (MAPK)活性的影响 ,探讨其在糖尿病肾病发病中的作用。方法 :采用人肾小球系膜细胞进行体外培养 ,按培养液中葡萄糖浓度分为对照组 (含 11mmol·L- 1 葡萄糖 )、高糖组 (含 3 0mmol·L- 1 葡萄糖 )、MAPK抑制剂组 (含 2 5 μmol·L- 1 PD980 5 9加 3 0mmol·L- 1 葡萄糖 )及甘露醇组 (含 2 0mmol·L- 1甘露醇 ) ,以32 P标记的髓磷脂碱性蛋白为底物 ,用同位素示踪技术测定培养细胞的MAPK活性。结果 :高糖组MAPK活性明显高于其它各组 ;加入MAPK抑制剂后 ,其活性明显降低 ,同时细胞的增殖受到抑制。结论 :高糖可增加人肾小球系膜细胞MAPK活性 。
Objective To study the effects of high glucose on activity of mitogen activated protein kinase (MAPK) in cultured human mesangial cells and to explain the mechanism of diabetic nephropathy. Methods The human mesangial cells were incubated and divided into five groups according to the concentration of glucose in medium: normal group (11-mmol·L -1 glucose); high glucose group (30-mmol·L -1glucose);the MAPK special inhibitor PD98059 group (25-μmol·L -1 PD98059+30-mmol·L -1 glucose) and mannitol group (20-mmol·L -1 mannitol) as osmolity control. We used the MBP marked by 32P as substance to test the activity of MAPK. Result The activity of MAPK in the high glucose group was higher than that in the other groups.When treated with PD98059 ,the cell proliferation was inhibited. Conclusion The activity of MAPK in human mesangial cells increases when treated with high glucose,which may be related with the stress-activation induced by osmolity.
出处
《东南大学学报（医学版）》
CAS
2003年第6期366-368,379,共4页
Journal of Southeast University(Medical Science Edition)
基金
铁道部科技基金资助项目(J99Z1 1 9)
