摘要
使用具有负有效淌度的十二烷基磺酸钠胶束赋予中性分子有效淌度 ,以中性主体分子 1 8-冠 -6和中性客体分子对 -硝基苯胺形成中性超分子包结化合物为实例 ,建立了中性分子相互作用的毛细管电泳分析方法 ,发现对 -硝基苯胺和 1 8-冠 -6与 SDS的相互作用存在相当的差异 .测得 1 8-冠 -6和对 -硝基苯胺的 lg K为4. 1 4,1 8-冠 -6与 2 -氨基吡啶、 1 8-冠 -6与 SDS以及 SDS与 2 -氨基吡啶的 lg K分别为 3 .65 ,3 .1 3和 1 .1 5 .
Because neutral molecules have no absolute mobilities, the capillary electrophoresis utilizing peak-shifting experiment can not be used to analyze the interaction of them. In this paper, the neutral molecules have been imparted apparent mobilities by sodium dodecyl sulfate(SDS) micelle, which has negative charge and negative apparent mobilities. The 18-crown-6 and p-nitroaniline without charge under a controlled condition were used as the samples of neutral molecules, and they were able to form a neutral supramolecule by interaction. Through the research, an interaction analytical method by using capillary electrophoresis is set up. The logarithm of binding constant of 18-crown-6 with p-nitroaniline was obtained to be 4.14. In addition, comparative differences of hydrophobicity between 18-crown-6 and p-nitroaniline to SDS was found, respectively. Further more, for multiple interactions, the interaction analytical method of supramolecules has also been set up, which has more accuracy and availability. The logarithms of binding constant of 18-crown-6 to 2-aminopyridine and SDS, and 2-aminopyridine to SDS were obtained to be 3.65, 3.13 and 1.15, respectively. After the development of the peak-shifting was applied to the neutral molecules, the principles, available experimental conditions, and assumptions in the measurement have been discussed. This approach in this paper could be developed as an interaction analysis method of multiple components in a complex system.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第11期1967-1971,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 0 175 0 3 8)
教育部跨世纪优秀人才培养计划资助
教育部高等学校青年教师奖 (批准号 :教人司 [2 0 0 2 ] 12 3 )资助
