摘要
Dig标记和检测试剂盒中的封阻试剂配制成0.05%、0.1%、0.3%、0.5%、0.7%、0.9%六种浓度,65～68℃温度时,溶解时间分别为10、15、20、35、40、45分钟;预杂交,在免疫测定中进行封阻,背景反应最小,其次是不预杂交,在免疫测定中进行封阻,再次是预杂交,在免疫测定中不封阻;标记探针保存在-20℃,至少可稳定18个月,同一探针重复使用三次可获得满意效果.以上结果表明,DigDNA标记和检测系统将代替^(32)p标记及其检测系统.
Blocking reagart in DIG DNA Labeling and Detection kit was made up 0.05, 0.1, 0.3, 0.5, 0.7, 0.9 percent, under the temperature of from 65℃ to 68℃,time of dissolution was 10, 15, 20, 35, 40, 45 minutes, respectively. If made prehybridization and blocked with blocking regent in immundogical de-tection(I.D.), the background was least, if not made prehybridization and blocked with blocking reagent in I.D., the background was Less, if made prehybridization and not blocked with blocking reaget in I.D., the background was a little. The labeled DNA probe was stable at-20℃ for at least 18 mouths and could be repeated three times to get satisfying results. The experiment results show that Dig DNA labeling and detection system will take the place of 32p DNA labeling and detection system.
出处
《生物技术》
CAS
CSCD
1992年第6期11-13,共3页
Biotechnology
关键词
DNA
杂交技术
核酸探针
Digoxigenin
DNA
Hybridization technique
