摘要
从猪垂体中提取出DNA,以EMBL3噬菌体作载体,构建了染色体基因文库。用全长的猪生长激素(pGH)cDNA作探针,经原位杂交,筛选出5个阳性克隆。提取其中一个阳性克隆的DNA,通过斑点杂交,酶解和Southorn印迹,表明它含有全长猪生长激素基因;将Sma Ⅰ酶解后Southorn印迹为阳性的两个片段进行了亚克隆,对其中含有猪生长激素基因Sma Ⅰ位点上游序列的片段进行了部分序列分析,并和已发表的相应序列进行了比较。
The high molecular weight DNA was isolated from porcine pituitary gland,and the genomic library was constructed using λ EMBL3 as the cloning vector. Five positive clones were identified by in situ hybridization with the full length pGH cDNA.Dot-blot hybridization,restriction analysis and Southern hybridization showed that one of the positive clones contains the whole sequence of pGH gene.Tow positive Sma I fragments were subcloned, one of which contains the upstream sequence from the Sma I site of pGH gene and subsequently were partly sequenced.We also compared the sequence that we obtained with the reported corresponding sequence of pGH gene.
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第4期318-323,共6页
Chinese Journal of Biotechnology
基金
博士点基金
关键词
猪垂体
序列分析
生长激素基因
Porcine pituitary gland
porcine growth hormone gene
DNA sequencing
