摘要
以携带营养缺陷型标记的季也蒙假丝酵母（Candida guilliermondii S 208 Arg-）和酿酒酵母（Saccharomyces cerevisiae 314）为亲本,采用GH-401型电诱导基因转移/细胞融合仪,用3个强度为18kV/cm,时程为10μs,间隔为1s的高压电脉冲处理原生质体,营养互补的融合频率为3.6×10-3。在选择和非选择培养基上连续传代20余次后,对得到的稳定的融合子进行分析比较,结果表明,它们是两亲株融合后形成的融合体。其中有两株融合子能利用木糖和纤维二糖生产乙醇,F-106利用D-木糖（2%）和纤维二糖（2%）生产乙醇的产量分别为3.1g/l和1.05g/l;F-308分别为0.2g/1和2.8g/l。
The proloplast fusion between Candida guilliermondii S208 (Arg^-)and Saccharomyces cerevisiae 314 was induced with the model GH-401 Electric Induced Gene Transfer/Cell Fusion System by three pulses (18kV/cm, 10μs duration) applied at an interval of 1s. The frequency of appearance of prototrophic hybrids was 3.6×10~3. Stable fusion products were obtained after continual subculture on selected and unselected medium more than 20 times. The results of com-parative studies showed that the fusion products were real hybrids of both parents.Two fustants,F-106 and F-308, could ferment both D-xylose(2%) and cellobiose (2%)to ethanol. The ethanol production of F-106 were 3.1g/1 and 1.05 g/1 respectively, F-308 were 0.2 g/1 and 2.8 g/1 respectiv-ely.
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第1期82-86,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目
关键词
D-木糖
纤维二糖
乙醇
酿酒酵母
季也蒙假丝酵母
Electric field-induced fusion
D-xylose
cellobiose
ethanol
Candida guilliermondii
Saccharomyces cerevisiae
