摘要
目的 建立一种同时鉴别金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌 (MRSA)、耐甲氧西林凝固酶阴性葡萄球菌 (MRCNS)的快速方法。方法 利用双重聚合酶链反应 (PCR)技术同时检测金黄色葡萄球菌特有的耐热核酸酶 (NUC)基因和编码耐甲氧西林葡萄球菌青霉素结合蛋白 (PBP2a)的mecA基因。结果 94株金黄色葡萄球菌 (SA)的NUC基因 10 0 %阳性 ,mecA基因阳性率为 4 8 9% ,药敏法MRSA的阳性率为 4 7 9% ;10 1株凝固酶阴性葡萄球菌 (CNS)的NUC基因 10 0 %阴性 ,mecA基因阳性率为 35 6 % ,药敏法MRCNS阳性率为 34 7%。结论 双重PCR技术检测葡萄球菌 (SA、CNS)及其甲氧西林耐药性具有简便、快速、敏感和特异的特点 。
OBJECTIVE To develop a rapid method for simultaneously identifying Staphylococcus aureus(SA), meticillin-resistant S. aureus (MRSA) and meticillin-resistant coagulase-negative Staphyloccus (MRCNS). METHODS Duplex polymerase chain reaction was developed for simultaneously detecting NUC gene, a unique gene for S. aureus, and mecA gene, encoding PBP2a for meticillin resistance. RESULTS The results demonstrated that all 94 tested strains of SA were positive for NUC gene, and 46 of 94 (48.9% ) strains positive for mecA gene. In antibiotic susceptibility assays, 47.9% (45/94) strains showed MRSA positive. For 101 strains of coagulase-negative Staphyloccus (CNS), 100% showed negative for NUC gene, 35.6%(36/101) positive for mecA gene. 34.7%(35/101) MRCNS was positive in antibiotic susceptibility assays. CONCLUSIONS The results proved that duplex PCR for detecting both Staphylococcus (SA, CNS) and its meticillin-resistance strains was a rapid, simple, sensitive and specific method. It can be used as an efficient diagnostic method.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第10期916-918,共3页
Chinese Journal of Nosocomiology
