摘要
应用单链构象多态性 (SSCP)和序列分析方法研究了来自我国 9个省份的水稻条叶枯病毒(RSV) 80个田间分离物的NS2基因遗传结构特征 .SSCP分析结果表明 ,我国RSVNS2基因遗传结构符合准种 (quasispecies)结构特征 .部分分离物的序列分析结果表明 ,RSV上述分离物和已报道的日本 2个分离物可以归入 2个组 :云南的部分分离物划分为 1个组 ;其它分离物及日本T、O的 2个分离物为另 1组 .组与组之间 ,NS2蛋白基因核苷酸同源性为 94 %～ 95 % ,氨基酸同源性为 95 %～ 97% .遗传多样性分析结果表明 ,RSV种群存在地理隔离但在种群间可能发生了基因漂移 (geneflow) .
The population structure and genetic variation of rice stripe virus (RSV) isolates were estimated by single strand conformation polymorphism (SSCP) and sequence analysis of the RSV NS2 gene. SSCP analysis of 80 isolates collected from 9 provinces in China showed that these isolates had a quasi species structure. Sequence analysis showed that these isolates and two Japan isolates could be divided into two diverged groups. A part of Yunnan isolates formed one group and the other group contained the rest of the RSV isolates. The two groups shared 94%—95% and 95%—97% sequence identities at the nucleotide and amino acid levels, respectively. Phylogenetic studies based on the sequences confirmed the geographical isolation of RSV virus population, but showed that gene flow may occur between populations. Negative selection caused by NS2 protein possible movement protein function and founder effect due to vector transmission were proposed to explain the intra and inter population diversity observed.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2003年第5期600-605,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金(No .3 0 0 0 0 0 0 2
3 0 2 40 0 17)
2 0 0 1年高等学校全国优秀博士学位论文作者专项资金项目(2 0 0 15 0 )
福建省自然科学基金(No.B0 110 0 14 )~~
