摘要
目的 探讨重症肌无力(MG)患者T细胞受体(TCR)β链(V β)亚家族优势表达及β链3号互补决定区(CDR3)的长度谱型特点;研究强的松治疗MG患者前后TCR V β优势表达及β链CDR3长度谱型特点的变化。方法 通过逆转录聚合酶链式反应扩增MG患者外周血循环T细胞中不同亚家族含CDR3的大片段,分析亚家族的表达,并经基因扫描(长度谱型分析)分析CDR3谱型。结果 MG患者外周血TCR Vβ 6、8、12、15亚家族呈倾斜性分布,且部分亚家族T细胞呈现单克隆或寡克隆增殖;强的松治疗后症状明显改善,倾斜性分布现象在缓解期逐渐恢复正常。结论 TCR V β 6、8、12、15亚家族可能与MG症状有关;强的松类肾上腺皮质激素药治疗MG的机制可能是通过改变TCR V β亚家族表达以及抑制病理性T细胞克隆增殖而发挥作用。
Objective To investigate the restricted usage of T cell receptor V β subfamily of the circulating T cells in patients with myasthenia gravis and the characteristics of CDR3 size spectratype and to analyze the effect of prednisone on V β usage and CDR3 spectratypes. Methods The levels of TCR V β 6,8,12,15 gene expression were analyzed by using RT-PCR , and CDR3 size spectratyping by genescan. Results The preferential expression of TCR V β subfamilies 6, 8, 12, and 15 were found in 41 out of 64 untreated MG patients. T cell clonality expansion (mono-or oligoclonal expansion) was observed among TCR V β subfamilies 6,8,12 and 15. The restricted usage of TCR V β subfamily in the group had disappeared after treating with prednisone and gradually returned to normal distribution on remission, as the myasthenic symptoms improved significantly. Conclusions TCR V β subfamilies 6,8,12 and 15 may be involved in MG pathogenesis, and prednisone may improve MG symptoms with changed expression of TCR V β subfamily gene or suppression of pathogenic T cell clonal expansions.
出处
《中国神经免疫学和神经病学杂志》
CAS
2003年第4期238-241,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
湖北省自然科学基金资助项目(992P0112)
