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诱生的NO对原代培养人甲状腺细胞的影响

The Effects of Inducible Nitric Oxide on Primary Cultured Human Thyrocytes
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摘要 目的 研究原代培养人甲状腺细胞在IL - 1α刺激下NO生成情况及NO对甲状腺细胞毒作用和对甲状腺功能影响。方法 IL - 1α和L -NAME刺激甲状腺细胞 4 8h ,测定培养液中NO含量和LDH活力以及细胞中TPO活性 ;用SNP刺激甲状腺细胞 4h ,测定甲状腺细胞碘有机化。结果 IL - 1α剂量依赖性诱导甲状腺细胞产生NO ;IL - 1α能提高甲状腺细胞LDH释放 ,降低TPO活性 ,L -NAME可抑制上述作用 ;SNP剂量依赖性抑制甲状腺细胞碘有机化。结论 IL - 1α能诱导甲状腺细胞产生大量NO ;NO对甲状腺细胞有部分细胞毒作用 ,可抑制TPO活性和甲状腺细胞碘有机化。 Objective To determine the induction of NO production by IL-1α in primary cultured human thyrocytes,and to study whether NO was involved in cytokine induced-cytotoxicity and a possible role of NO in regulation of thyroid function.Methods Cultured human thyrocytes were stimulated by IL-1α and L-NAME 48h,NO(measured as NO 2)and cytosolic LDH contents in medium,and TPO activity were respectively measured.After exposed to SNP for 4h,iodine organification was measured.Results IL-1α substantially increased NO formation in a dose-dependent fashion,the treatment significantly increased the release of LDH in the medium and decreased intracellular TPO activity,the effects were blocked by L-NAME,SNP also inhibited iodine organification in a dose-dependent fashion.Conclusions Human thyrocytes produce NO in response to IL-1α,NO acts as a mediator of cytokine induced-cytotoxicity and plays a role in inhibiting TPO activity and iodine orgnification.
出处 《锦州医学院学报》 2003年第4期22-25,共4页 Journal of Jinzhou Medical College
关键词 原代培养 甲状腺细胞 一氧化氮 甲状腺过氧化物酶 桥本氏甲状腺炎 甲状腺肿瘤 thyrocytes nitric oxide cytotoxicity thyroid peroxidase iodine orgnification
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