摘要
目的 :采用高效毛细管区带电泳法对如意金黄散 (姜黄、大黄、黄柏、苍术等 )中的盐酸小檗碱及盐酸巴马亭进行定量测定。方法 :采用HP3D全自动毛细管电泳仪对如意金黄散中盐酸小檗碱及盐酸巴马亭的含量进行定量测定。电泳条件为 :石英毛细管柱 10 0 μm× 5 6 .5cm(有效长度 4 8cm) ;缓冲液为 10mmol·L-1混合磷酸盐溶液 ( pH =4 .8) ,含 30 %乙腈 ;分离电压 2 0kv ;检测波长 :2 30nm ;毛细管温度为 30℃。结果 :盐酸小檗碱和盐酸巴马亭在 5 μg·mL-1～ 10 0 μg·mL-1范围内呈良好的线性关系 ,其回归方程分别为Y =0 .0 2 0 96 4X - 0 .0 0 771(r =0 .9998)和Y =0 .0 10 92X - 0 .0 2 939(r =0 .9997) ;平均回收率分别为 98.7% (n =5 ,RSD =1.82 % )和 10 2 .1% (n =5 ,RSD =2 .81% )。结论 :本法简单、快捷、灵敏。
Objective: A capillary electrophoresis for the determination of berberine hydrochloride and palmatine hydrochloride in Ruyijinhuang Powder(Rhizoma Curcumae Longae, Radix et Rhizome Rhei, Cortex Phellodendri, Rhizoma Atractylodis, etc.) was established. Methods: The berberine hydrochloride and palmatine hydrochloride were determined using benzoic acid as an internal standard its detection wavelength was at 230nm. The optimized condition was achieved with a fused\|silica capillary 100μm×56.5cm, 10mmol·L -1 phosphate buffer(pH=4.80) containing 30% acetonitrile, a constant voltage of 20kv and temperature at 30℃. Results: Calibration curve was constructed in the range of 5μg·mL -1 ～100μg·mL -1 for berberine hydrochloride and palmatine hydrochloride, respectively. The regression equations were Y=0.02964X -0.00771 ( r =0.9998) and Y =0.01092 X -0.02939 ( r =0.9997) for berberine hydrochloride and palmatine hydrochloride, respectively. The average recovery was 98.7% and 102.1%( n =5), respectively. Conclusion: This method is sample, quick and sensitive.
出处
《中成药》
CAS
CSCD
北大核心
2003年第11期877-879,共3页
Chinese Traditional Patent Medicine
