摘要
目的观察严重烧伤病人接受重组人生长激素（ｒｈＧＨ）治疗后血清生长激素（ＧＨ）、胰岛素样生长因子－１（ＩＧＦ－１）、创面ＤＮＡ合成。细胞增殖活性及表皮生长因子受体（ＥＧＦＲ）表达的变化，探讨生长激素促进烧伤创面愈合的机制。方法３３例严重烧伤病人随机分成ｒｈＧＨ组（１８例）和对照组（１５例）。ｒｈＧＨ组每晚１０点接受ｒｈＧＨ皮下注射（０．１ｍｇ· ｋｇ^(－１)），疗程 １４ｄ。ＥＬＩＳＡ检测血清 ＧＨ和 ＩＧＦ－１浓度；流式细胞计检测细胞 ＤＮＡ增殖指数（ＰＩ）和合成期细胞百分数（ＳＰＦ）；免疫组化学检测增殖细胞核抗原（ＰＣＮＡ）。ＥＧＦＲ、细胞角蛋白（ＣＫ）的表达。结果ｒｈＧＨ组血清ＧＨ、ＩＧＦ－１浓度较高，创面ＰＩ、ＳＰＦ明显高于用药前和对照组。免疫组化显示ＰＣＮＡ、ＥＧＦＲ、ＣＫ的表达明显增加。结论ｒｈＧＨ可以提高严重烧伤病人血清ＧＨ、ＩＧＦ－１水平，促进烧伤创面细胞ＤＮＡ合成和上皮细胞增殖，增加 ＥＧＦＲ表达，加速创面上皮化，促进烧伤创面愈合。
Objective To investigate the mechanism of recombinant human growth hormone (rhGH) in acceleration of burn wound healing.Methods Thirty three severe burn patients were enrolled in this study and randomly divided into rhGH group (n=18) and placebo control group (n=15). The rhGH was administered subcutaneously in the dose of 0.1 mg·kg^(-1) at 10 pm every evening in rhGH group for 14 days, while normal saline was used as a placebo in the control group. The serum concentration of GH and IGF -1 were assayed with EUSA method, and the proliferation index (PI) and S-phase fraction (SPF) of wound tissue was analyzed by flow cytometry (FCM). The expression of PCNA, EGFR and CK was assessed by immunohistochemical stains. Results Serum concentrations of GH and IFG -1 increased significantly after rhGH administration. The PI and SPF of wound tissue were obviously higher than those before rhGH administration and those in the control group. The expressions of PCNA, EGFR and CK was enhanced. In addition, the epidermis of healed wound was thicker in rhGH group than that in the control group. Conclusion The application of rhGH in burn patients could improve the serum concentration of GH and IFG -1, promote DNA synthesis and PGNA and EFGR expression of epithelium, so that re-epithelialization of the burn wound could be accelerated.
出处
《中华烧伤杂志》
CAS
CSCD
2000年第1期22-25,共4页
Chinese Journal of Burns
