摘要
目的 观察抗CD4 9d单克隆抗体 (McAb)联用重组人粒细胞集落刺激因子 (rhG CSF)动员的外周血干细胞 (PBSC)救治放射损伤小鼠的效果。方法 8 5Gy6 0 Coγ射线照射的BALB c小鼠 ,分别接受经rhG CSF(1组 )、抗CD4 9dMcAb(2组 )、rhG CSF联合抗CD4 9dMcAb(3组 )动员的PBSC移植。并以生理盐水组为对照 ,观察受体小鼠的 4周存活率、外周血白细胞 (WBC)、骨髓有核细胞(BMNC) ,粒细胞巨噬细胞集落形成单位 (CFU GM)及脾集落形成单位 (CFU S)等指标。结果 实验 3组小鼠的 4周存活率、WBC、BMNC ,CFU GM、CFU S数均明显高于对照组、1组和 2组 ,差异具有显著性或非常显著性 (P <0 0 5或P <0 0 1)。结论 抗CD4 9dMcAb联合rhG CSF使用能协同动员小鼠PBSC ,并能成功救治放射损伤小鼠。
Objective To study the rescue effects of peripheral blood stem cell (PBSC) mobilized by anti-CD49d monoclonal antibody (McAb) combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF) on lethally irradiated mice. Methods BALB/c mice received 8.5 Gy total body irradiation were transplanted with either NS (control group),PBSCs from rhG-CSF-mobilized mice (experimental group 1),PBSCs from anti-CD49d McAb-mobilized mice (experimental group 2),or PBSCs from anti-CD49d McAb and rhG-CSF-combinatively mobilized mice (experimental group 3).The white blood cells count,4-week survival rate,and numbers of bone marrow nucleated cells (BMNC),granulocyte-macrophage colony-forming unit (CFU-GM),colony-forming unit-spleen (CFU-S) were observed. Results The survival rate and WBC,BMNC,CFU-GM,CFU-S counts were all significantly higher in experimental group 3 than those in the control group,experimental group 1,and experimental group 2 ( P <0.01 or P <0.05). Conclusions Anti-CD49d McAb and rhG-CSF may synergetically mobilize PBSC in mice.PBSCs mobilized by combination of anti-CD49d McAb and rhG-CSF could effectively rescue lethally irradiated mice. ;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2003年第5期334-336,共3页
Chinese Journal of Radiological Medicine and Protection
基金
济南军区"十五"重点科研项目 (0 2Z49)
关键词
抗CD49dMcAb
rhG—CSF
PBSC
救治
放射损伤
小鼠
Anti-CD49d monoclonal antibody
Granulocyte-colony stimulating factor
Mobilization
Hematopoietic stem cell
Radiation injury
