摘要
目的 探讨肝纤维化过程中血小板衍生生长因子(PDGF)和转化生长因子 (TGF β1)表达的动态变化。方法 采用CCl4诱导的大鼠肝纤维化模型 ,分别于造模的第 3、7、10周末处死动物 ,取肝组织石蜡包埋 ,制作组织芯片 ,免疫组织化学SP法检测PDGF、TGF β1的改变。结果 正常肝组织不表达或微弱表达PDGF、TGF β1,纤维化的肝组织PDGF、TGF β1表达明显增强 ,并随肝纤维化时间的延长而增强 (P <0 0 5 ) ,第 7、10周间表达差异无显著性。两者的表达呈正相关。结论 PDGF和TGF
Objective To study the dynamic changes in the expression of platelet-derived growth factor (PDGF) and transforming growth factor beta -1 (TGF-β 1) in the development of hepatic fibrosis. Methods Carbon tetrachloride (CCl 4) induced rat hepatic fibrosis was used. The rats were killed at the en d of the 3rd, 7th and 10th week after the initial administrations of CCl 4, and the liver tissues were then used to make tissue microarrays (TMA). Immunohistochemical SP method was used to detect the expression of PDGF and TGF-β 1 proteins. Results Normal liver tissue did not express or weekly expressed PDGF and TGF-β1, while fibrotic liver tissue expressed the two cytokines increasingly with the development of liver fibrosis. Positive correlation was found between the expression of PDGF and that of TGF-β 1 in the fibrotic liver tissue. Conclusion Both PDGF and TGF-β 1 are important cytokines in the pathogenesis and development of hepatic fibrosis.
出处
《安徽医科大学学报》
CAS
2003年第5期340-342,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学研究基金资助课题 (编号 :990 44 12 6)
