摘要
目的:观察牛磺酸对四氯化碳(CCl_4)诱导大鼠肝纤维化的保护及其机制.方法:采用CCl_4诱导的大鼠肝纤维化模型,研究牛磺酸(400 and 800 mg/kg per day for 12 weeks)对血清丙氨酸氨基转移酶(ALT)、血浆蛋白、透明质酸(HA)、Ⅲ型前胶原(PC Ⅲ)及肝组织羟脯氨酸(HYP)、肝药酶和抗转化生长因子β_1(TGF-β_1)的影响,并观察肝组织病理学变化.结果:牛磺酸能明显减轻纤维化程度,降低ALT(2 601±300→1367 ± 317 nkat/L,P<0.01),HA(324 ± 95→219 ± 79 μg/L,P<0.01),PC Ⅲ(33 ± 14→19± 8μg/L,P<0.01)和HYP(1 282 ± 523→381±147μg/L,P<0.01)水平,提高肝细胞色素P450(Cyt.P450)(211 ± 27→811±136 nmol/g,P<0.01),细胞色素b_5(Cyt.b_5)(256 ±69→761±131 nmol/g,P <0.1)和血清白蛋白含量(23±3→31 ±4 g/L,P<0.05),并明显减少TGF-β_1的表达(33±13→20 ± 6,P<0.01).结论:牛磺酸具有显著的抗CCl_4诱导的大鼠肝纤维化作用,其作用机制可能与减轻肝细胞损伤和抑制TGF-β_1的表达有关.
AIM: To study the protective effect of taurine on hepatic fibrosis induced by carbon tetrachloride (CCl_4) in rats and its mechanisms. METHODS: CCl_4-induced rat hepatic fibrosis was treated by taurine (400 and 800 mg/kg per day for 12 weeks), and serum alanine aminotransferase (ALT), plasma protein, hyaluronic acid (HA) and procollagen Ⅲ (PC Ⅲ), and hepatic hydroxyproline (HYP), hepatic microsomal drug-metaboliz- ing enzyme and anti-transforming growth factor β_1(TGF-β_1) were measured. In addition, pathological changes of liver tissue were observed by light microscope. RESULTS: The hepatic fibrosis, activity of serum ALT (2601±300→1367±317 nkat/L, P<0.01), and contents of serum HA (324±95→219±79 μg/L, P<0.01), PC Ⅲ (33±14→ 19±8 mg/L, P<0.01) and hepatic HYP (1282±523→ 381±147 μg/L, P<0.01) were markedly reduced by the treatment with taurine (32.57±13.73 vs 19.20±8.25 ng/ml, P<0.01 and 1.282±0.523 vs 0.463±0.159 μg/ml, P<0.05), The contents of plasma albumin (23±3→31±4 g/L, P<0.05) and hepatic cytochrome P450(Cyt.P450)(211±27→ 811±136 nmol/g, P<0.01) and cytochrome b_5 (Cyt.b_5) (256±69→761±131 nmol/g, P<0.01) were increased by the same treatment (0.211±0.027 vs 0.765±0.106 nmol/mg protein, P<0.01 and 0.256±0.069 vs 0.697±0.120 nmol/mg protein, P<0.01). In addition, taurine could also significantly inhibit the expression of TGF-β_1(33±13→20±6, P<0,01), CONCLUSION: CCl_4-induced hepatic fibrosis is markedly relieved by the treatment of taurine, and its protective mechanisms are related to the decrease in liver injury and expression of TGF-β_1.
出处
《世界华人消化杂志》
CAS
2003年第9期1392-1395,共4页
World Chinese Journal of Digestology
基金
广西壮族自治区自然基金
No.0144016
