摘要
目的 检测基质金属蛋白酶MMP-2、-9及其抑制剂TIMP-2、-1在不同分期神经母细胞瘤中的表达,探讨它们在神经母细胞瘤浸润转移中的可能机制及在治疗中的指导意义。方法 应用免疫组化SABC法检测35例神经母细胞瘤中MMP-2、-9及TIMP-2、-1的表达。结果 MMP-2在晚期(Ⅲ、Ⅳ期)神经母细胞瘤中的强阳性表达率为79.2％,相对于早期(Ⅰ、Ⅱ、ⅣS期)的18.2％明显升高(P=0.001<0.01)。MMP-9在晚期和早期的强阳性表达率分别为79.2％和36.4％,也具有统计学意义(P=0.017<0.05)。TIMP-2的表达与MMP-2负相关,在早期以强阳性表达为主,在进展期以弱阳性为主(P=0.027<0.05)。结论 MMP-2、-9可促进神经母细胞瘤的浸润转移,而TIMP-2对二者有抑制作用。
Objective To detect the expression of MMP-2、-9、TIMP-2、-1, in different clinical
stage of neuroblastoma (NB), and the meaning in metastasis and prognosis of the tumor. Methods From
1990 to 2001, the specimens from 35 cases ( male 22, female 13) during NB resection were fixed in
formaldehyde and embedded in paraffin. According to INSS classification, 11 cases were belonged to early
stage(Ⅰ、Ⅱ、Ⅳs stage) and 24 cases to advanced stage ( Ⅲ, Ⅳ stage). Expressions of MMP-2、-9、TIMP-
2、-1 of specimens were studied by immunohistochemistry SABC method. Results Of the Ⅰ、Ⅱ、Ⅳs stage,
the strong expression of MMP-2 was 18.2%, and Ⅲ、 Ⅳ stage was 79.2% (P = 0.001008). While
MMP-9 staining was from 36.4% to 79.2% (P = 0. 016809). There were 9 samples with strong staining
of TIMP-2 in early stage, and 10 in advanced stage (P = 0. 026569). Conclusions The positive staining of
MMP-2、-9 is increased as the progression of NB, while TIMP-2 is presented with negative correlation. An
early detection may provide information for the efficacy of treatment.
出处
《中华小儿外科杂志》
CSCD
北大核心
2003年第5期452-454,共3页
Chinese Journal of Pediatric Surgery
基金
国家自然科学基金资助课题(编号30170974)
