摘要
目的 定量检测转化生长因子 β1(TGF β1)和TGF β2在大鼠正常肝脏中肝细胞和汇管区间质细胞中的表达水平。方法 激光显微切割技术分离大鼠正常肝脏中的肝细胞和结缔组织细胞 ,实时荧光定量聚合酶链反应 (PCR)技术分析TGF β1和TGF β2的mRNA含量。 结果 激光显微切割技术成功地将肝脏结缔组织细胞从肝细胞中分离。TGF β1的mRNA含量在肝细胞中是TGF β2的 11倍左右 ,在肝脏汇管区间质细胞中接近TGF β2的 5倍左右。 结论 激光捕获显微切割技术结合实时荧光定量PCR技术能够针对性地精确分析不同的和极少量的细胞中基因表达 ,在肝脏中TGF β1的合成多于TGF β2。
Objective To quantitatively detect the expression level of transforming growth factor β1 (TGF β1) and TGF β2 genes in the hepatocytes and non parenchymal cells around the portal vein of normal rat liver.Methods The connective tissue non parenchymal cells were separated from hepatocytes of the liver by laser microdissection.The mRNA level of TGF β1 and TGF β2 was detected quantitatively by the method of quantitative real time PCR (QRT PCR).Results The non parenchymal cells were successfully separated from the abundant hepatocytes.The mRNA level of TGF β1 was about 11 times in hepatocytes as that of TGF β2.Whereas in non parenchymal cells the mRNA level of TGF β1 was approximate 5 times as that of TGF β2.Conclusion Combination of LCM and QRT PCR could specifically and accurately detect gene expression level in different cells.In liver the TGF β1 gene was expressed more abundantly than TGF β2 and the expression level of two genes was different between hepatocytes and connective tissue non parenchymal cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第10期890-892,共3页
Chinese Journal of Experimental Surgery
