摘要
探讨内源性硫化氢 (H2 S)在感染性和内毒素性休克大鼠血管组织中的含量变化及意义。用盲肠结扎穿孔法制备大鼠感染性休克模型和静脉注射内毒素法制备内毒素休克大鼠模型 ,观察大鼠血液动力学、代谢变化及测定内源性硫化氢含量和一氧化氮含量 ,并计算其相关性。感染性及内毒素性休克大鼠的血液动力学参数均明显低于对照组 ,而左室舒张末压明显高于对照组 (P <0 0 1) ,血糖明显低于对照组 (P <0 0 1) ,血乳酸水平明显高于对照组(P <0 0 1)。感染性和内毒素性休克大鼠动脉组织中H2 S含量明显高于对照组 ,(均P <0 0 1)。休克大鼠血管H2 S含量与血压、心功能及低血糖的程度呈高度负相关 (均P <0 0 1)。以上结果说明内源性H2
The aim of the study was to explore the changes of H 2S in vascular tissues of rats with septic shock and endotoxin shock. A rat model of septic shock induced by cecal ligation and puncture and a model of endotoxic shock induced by injection endotoxin were developed to study the changes of endogenous H 2S in vascular tissues of rats during shock. Also, we measured hymodynamic variations, metabolic data, H 2S and nitric oxide (NO) contents of arteries. Hemodynamic parameters including heart rate (HR), mean arterial pressure ( BP), and the +dP/dt max decreased significantly while the left ventricular end diastolic pressure (LVEDP) increased significantly.The rats had hypoglycemia and severe lactic acidosis. H 2S contents in arteries were significantly increased in rats with septic shock and endotoxic shock. Endogenous vascular H 2S contents were negatively correlated with BP, cardiac function and the degree of hypoglycemia ( P <0.01). Endogenous vascular H 2S increased in rats with septic shock and endotoxic shock. It was suggested that H 2S play a role in physiological and pathophysiological process during the shock.
出处
《基础医学与临床》
CSCD
北大核心
2003年第4期384-387,共4页
Basic and Clinical Medicine
基金
国家重点基础研究发展计划 (G2 0 0 0 0 5 6 90 5 )
985北京大学心血管重点研究项目
