摘要
目的 :了解长春地区戊型肝炎病毒 ( HEV)株的变异程度。方法 :用逆转录 -套式 -聚合酶链反应方法从患者血清中获取病毒 ,用双脱氧终止法进行核苷酸序列分析。结果 :China Changchun-2 2 0 MC与日本株、猪的 HEV分离株 ( AF0 82 843)、美国株 - 1 ( AF0 60 669)、墨西哥株 ( M745 0 6)、第四基因型 ( AJ2 72 1 0 8)、缅甸株 ( M732 1 8)、中国株 ( M941 77、D1 1 0 93、D1 1 0 92 )、尼泊尔株 ( AF0 5 1 830 )、巴基斯坦株 ( M80 5 81、AF1 85 82 2 )核苷酸同源性为 72 %～ 88% ,氨基酸同源性为 93%～ 97%。结论 :China Changchun- 2 2 0 MC株与其它 HEV株相比有较高的基因异质性 ,可能是一新型的
Objective:To investigate the variation degree of hepatitis E virus strains from Changchun city China.Methods:Reverse transcription Nested polymerase chain reaction (RT Nested PCR) was used to isolate hepatitis E virus from serum of patients with acute hepatitis.Doubledeo xytermination was used to perform nucleotide sequence analysis.Results:Compared with SWINE HEV(AF082843),USA US 1 (AF060669),Mexico(M74506),Genotype4(AJ272108),Burma B1(M73218),China strains(M94177,D11093,D11092),Nepali(AF051830),Pakistan SARSS(M80581, AF185822),nucleotide homology of ChinaChangchun 220MC were, respectively, 72%~88% and amino acid homology were 93%~97%.Conclusion:Compared with other hepatitis E virus strains, ChinaChangchun 220MC has more gene heterogeneity and may be a new HEV strain. [
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第5期647-650,共4页
Journal of Jilin University：Medicine Edition
