摘要
目的 :探讨细胞外液酸碱度 ( p H0 )的改变对主动脉平滑肌细胞电生理特性的影响。方法 :通过改变培养的乳鼠主动脉平滑肌细胞外液的酸碱度 ,应用全细胞膜片钳技术记录细胞膜的变化和钾离子通道活动的情况。结果 :p H0 降低时引起细胞膜超极化 ,外向钾电流增大 ;反之 ,p H0升高时引起细胞膜去极化 ,外向钾电流减小。这种变化可以被 TEA阻断 ,而对 4- AP不敏感。结论 :p H0 改变引起主动脉平滑肌细胞膜电位及外向电流幅值变化 。
Objective: To study on the pH 0 effects of changes on membrane potential and K + channels activity in aortic smooth muscle cells. Methods: The extracellular fluid pH 0 of cultivated aortic smooth cells was changed, then the membrane potential and K + currents were recorded with whole cell patch clamp technique. Results: The membrane potential hyperpolarized and outward K + currents increased with the decreasing pH 0. On the contrary, the membrane potential depolarized and outward K + currents decreased with the elevating pH 0 and the changes could be blocked by TEA but not by 4 AP. Conclusion:The changes of membrane potential and K + channels in aortic smooth muscle cells induced by pH 0 may be connected with the open activity of the Ca activated K + channels. [
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第5期551-554,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题 (3980 0 2 2 6 )
关键词
细胞外隙
酸碱平衡
主动脉平滑肌
细胞学
电生理学
膜片钳术
乳鼠
Extracellular space
Acid base balance
Aorta/cytology
Muscle, smooth, blood vessel/physiology
Patch clamp technique
Electrophysiology
