摘要
目的:通过高脂膳食诱导肥胖大鼠模型,观察抗阻运动、有氧运动以及抗阻运动与有氧运动相结合3种运动方式对肥胖大鼠骨骼肌PGC-1α/FNDC5/PPARγ信号通路的影响,以及该通路在运动调控体重中的作用机制。方法:8周龄雄性SD大鼠适应性喂养1周后,随机分为正常对照组(CON组,8只)和肥胖造模组,肥胖组造模成功后,从中随机选取32只分为肥胖对照组(OC组)、肥胖有氧运动组(OAE组)、肥胖抗阻运动组(ORE组)、肥胖抗阻运动+有氧运动组(OARE组),每组8只。有氧运动采用跑台方式进行,最大强度20m/min。抗阻运动采用负重爬梯方式,适应性训练1周后,以大鼠体重的20%为初始负重,逐渐递增,最大负重量达大鼠体重的100%,隔天训练,总训练时间为12周。OARE组采用跑台和爬梯交替进行,强度与OAE组和ORE组相同。采用实时荧光定量PCR测定骨骼肌PGC-1α、FNDC5及PPARγ的mR NA相对表达量;WesternBlot法测定骨骼肌PGC-1α、FNDC5以及PPARγ蛋白的表达量。结果:1)经过12周的运动干预后,肥胖抗阻运动组、肥胖有氧运动组、肥胖抗阻运动+有氧运动组大鼠的体重都显著低于肥胖对照组(P<0.05),并且肥胖有氧运动组和肥胖抗阻运动+有氧运动组大鼠的体重显著低于肥胖抗阻运动组(P<0.05);2)肥胖有氧运动组大鼠的体脂率显著低于肥胖对照组(P<0.05),肥胖抗阻运动组以及肥胖抗阻运动+有氧运动组大鼠的体脂率低于肥胖对照组,高于肥胖有氧运动组,但都没有显著性差异(P>0.05);3)肥胖抗阻运动组、肥胖有氧运动组以及肥胖抗阻运动+有氧运动组骨骼肌PGC-1α、FNDC5、PPARγ的mR NA和蛋白表达量都显著高于肥胖对照组(P<0.05);肥胖有氧运动组骨骼肌PGC-1α、FNDC5、PPARγ的mR NA和蛋白表达量显著高于肥胖抗阻运动组以及肥胖抗阻运动+有氧运动组(P<0.05),肥胖抗阻运动+有氧运动组骨骼肌PGC-1α、FNDC5、PPARγ的mR NA和蛋白表达量显著高
Objective: To explore the effects of resistance exercise, aerobic exercise or resistance+aerobic exercise on PGC-1α/FNDC5/PPARγ signaling pathway in skeletal muscle of obese rats and its mechanisms in exercise regulating body weight. Methods: Diet-induced obese SD rats were randomly divided into obesity control group(OC group), obesity aerobic exercise group(OAE group), obesity resistance exercise group(ORE group) and obesity resistance+aerobic exercise group(OARE group), 8 rats in each group. The aerobic exercise was performed by running on an animal treadmill, the maximum speed was 20 m/min. The resistance exercise was performed by using a load climbing ladder, after one-week adaptive training, the initial load was set as 20% of the rats’ weight, the load was gradually increased to the maximum of 100% of the rats’ weight. The resistance exercise was trained every two days for 12 weeks. In OARE group, the aerobic and resistance exercise were conducted alternately, and the intensity was the same as those in OAE and ORE groups. The mR NA and protein expression levels of PGC-1α, FNDC5 and PPARγ were detected by qRTPCR and Western Blotting. Results: 1) After exercise training, the body weight of 3 exercise groups were significantly lower than the OC group(P<0.05),and the OAE and OARE groups were significantly lower than the ORE group(P<0.05);2) The body fat percentage of the OAE group was significantly lower than that of the OC group(P<0.05), the body fat percentage of the ORE and OARE groups were lower than that of the OC group but higher than the OAE group with no significant difference(P>0.05);3) The relative expression levels of PGC-1α, FNDC5 and PPARγ mRNA and protein in the ORE, OAE, OARE groups were significantly higher than that of the OC group(P<0.05);the relative expression levels of PGC-1α, FNDC5 and PPARγ mRNA and protein in the OAE group were significantly higher than the ORE and OARE groups(P<0.05);the relative expression levels of PGC-1α, FNDC5, and PPARγ mR NA and protein in the ORE gro
作者
邓红
徐晓阳
张卫
DENG Hong;XU Xiaoyang;ZHANG Wei(South China Normal University,Guangzhou 510631,China;Guangzhou Polytechnic of Sports,Guangzhou 510650,China)
出处
《体育科学》
CSSCI
北大核心
2019年第3期74-80,共7页
China Sport Science
基金
广州市教育局第二批协同创新重大项目(1201650010)
广东省体育局科研项目(GDSS2018N062)
