摘要
目的 :探讨中草药抗糖尿病发病和降血糖的机制。方法 :将大白鼠分成 5组 ,芪苓汤组、八味地黄丸组、柴苓汤组 ,分别接受相应药物治疗 ;模型组接受自来水治疗。各组从实验的d 8起腹腔内注射链脲霉素 30mg·kg-1,每天 1次 ,连续 5d。另设正常对照组 ,不作任何处理。检测大白鼠的体重、血糖、果糖胺、胰岛素及T淋巴细胞 (W3 2 5和OX8)的百分率 ,取胰腺组织作组织学检查。结果 :用药各组的糖尿病发病率比模型组低 (P <0 .0 5 )。d 2 6八味地黄丸组和柴苓汤组的血糖分别比d 15下降 2 5 %和 18% (P <0 .0 5 )。芪苓汤组和八味地黄丸组的W 3 2 5细胞比率明显高于对照组。用药各组的平均胰岛面积和平均 β细胞面积较模型组大。 结论 :八味地黄丸、柴苓汤方剂具有降血糖作用 ;3方剂不同程度降低糖尿病发病率可能与提高细胞免疫功能有关。
AIM: To investigate the mechanisms of Chinese herbal medicin e formulas (Qi-ling-tang, Ba-wei-di-huang-wan and Chai-ling-tang) in the p revention and treatment of diabetes. METHODS: Wistar rats were d ivided into five groups and treated with Qi-ling-tang (the group QLT), Ba-wei -di-huang-wan (the group BWDHW), Chai-ling-tang (the group CLT), water (the group model) and control, respectively. The rats in groups QLT through model we re injected with 30 mg·kg -1 /daily streptozotocin (STZ) for five days beginning on day 8. The glucose concentration, fructosamine, insulin, total-pr otein, albumin and the percentage of W3/25 and OX8 T-cells in serum as well as the body weight of the rats were measured. Tissue sections of paraffin-embedde d pancreas of the rats were observed after staining with HE, AZAN and immunohist ochemistry. RESULTS: After STZ injection, the diabetic incidence of the rats in groups QLT, BWDHW and CLT were significantly lower than that in the group model, especially in the group BWDHW was only 21% by study day 10. A significant decrease in blood glucose concentration was found in groups BWDHW (25%) and CLT (18%) from study day 15 to 26. The percentage of W3/25 cell s in groups QLT and BWDHW was higher than that in the control group. The averag e area of islets and the area of bate cells were greater in groups QLT, BWDHW an d CLT than in the group model. CONCLUSION: Three formulas retard the development of hyperglycemia of rats induced with multiple low doses STZ by increasing the cell mediated immunofunction. The formulas Ba-wei-di-huang-w an and Chai-ling-tang can reduce hyperglycemia.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第6期680-685,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
糖尿病鼠
链脲霉素
中草药
细胞免疫
免疫组织化学
pharmacodynamics
diabetic rats
streptozotocini
herbal medicine
cell-mediated immunity
immunohistochemistry
