摘要
目的 :构建三种重构型人caspase - 8基因的原核表达载体 ,转染大肠杆菌并诱导其表达。方法 :将人caspase- 8催化结构域基因及两种大、小亚基基因次序颠倒的重构型人caspase - 8基因克隆入原核表达载体pBV2 2 0 ,转染大肠杆菌并诱导表达。结果 :成功构建了三种重构型人caspase- 8基因的原核表达载体。转染大肠杆菌后 ,经温度诱导 ,重构型人caspase - 8基因获得了高效的表达。结论 :在大肠杆菌中成功表达了三种重构型人caspase - 8基因。
Objective:To construct three recombinant human caspase-8 genes into prokaryotic expression vectors and express them in E.coli.Methods:Subcloned the catalytic domain gene of human caspase-8 and two reconstructed human caspase-8 genes in which small subunits were settled prior to large subunits into prokaryotic expression vector pBV220 and induced their expression in E.coli.Results:Three prokaryotic expression vectors carrying recombinant human caspase-8 genes were constructed successfully and expressed efficiently in E.coli.Conclusion:Three recombinant human caspase-8 genes were expressed in E.coli successfully.
出处
《西北国防医学杂志》
CAS
2003年第5期364-366,共3页
Medical Journal of National Defending Forces in Northwest China
