摘要
研究了以庆大霉素生产菌绛红小单孢菌为供试菌株,比较了低能N+离子、紫外线和60Co γ 射线对绛红小单孢菌的剂量存活效应和诱变效果。结果发现,N+离子注入的存活曲线与紫外线、60Co γ 射线辐照不同,即紫外线,60Co γ 射线照射绛红小单孢菌的剂量存活曲线均是指数曲线,而N+离子注入呈复杂下降、上升、再下降的趋势,而且3种辐射在诱变效应上也有很大的差异。在相同死亡率条件下,紫外线诱变率低,但正变多于负变,而 γ 射线诱变率高,负变远远多于正变。适当剂量的N+离子注入表现出诱变率高、正变率高及正变幅度大的特点,效果更像紫外+LiCL的复合诱变。并确定了N+离子注入绛红小单孢菌的适宜诱变剂量是在存活率高峰处附近。
The effect of 30 keV N+ ions, UV and 60Co γ-rays on the survival and the gentamycin yield of Micromonospora purpurea were investigated. A saddle-like survival curve was found Micromonospora purpurea treated with N+ ions, whereas the curves of those irradiated by UV and 60Co γ-rays were exponential, Comparisons of the induction indexes indicated that the N+ irradiation was more advantageous than UV and the γ-rays. Based on the experiments, it is concluded that induction doses of 0.5～1×1016 /cm2, i,e. the doses correlating to the peaks of the survival curve, are suitable for high yield of gentamycin.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2003年第2期120-124,共5页
Journal of Radiation Research and Radiation Processing
基金
国家95重大科技攻关项目(96-538-01-01-09)
