摘要
以胡杨和柽柳的叶片为材料 ,建立了一种从植物组织中快速分离总RNA的方法。该方法具有三个显著的特点 :RNA提取周期短 ,应用改进的LiCl沉淀RNA方法 ,仅需 10min即可 ;方法简单易行 ,仅需要几种常用试剂 ,操作步骤简单 ;提取的RNA杂质少 ,得率高 ,并能在提取过程中较有效抑制RNA的褐化效应。RNA质量可以满足cDNA文库构建、DDRT -PCR等要求。
A method of rapid isolation of total RNA was developed by using fresh leaves of Tamarix and Populus euphratica Oliv as test materials. This method has three obvious advantages: the cycle of isolation is short, if using improved method of RNA deposition by LiCl it only need 10 minute to deposit RNA; it is simple and only need some kinds of common reagents; isolated RNA has high purity and browning effect can be inhibited by this method. The qualities of RNA can meet the demands of cDNA library construction and DDRT-PCR reaction.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2003年第5期99-100,共2页
Journal of Northeast Forestry University
基金
国家"973"树木育种的分子基础资助项目 (G1 9990 1 60 0 0 )
