摘要
目的 探讨己酮可可碱 (pentoxifylline ,PTX)对淡水淹溺肺损伤后肺组织细胞间粘附分子 (ICAM 1 )、血管间粘附分子 (VCAM 1 )表达的影响。方法 1 8只普通杂种犬随机分为对照组、淹溺组和淹溺加己酮可可碱组三组。采用气管切开插入Carlen’s管分隔左右两肺 ,向右肺灌淡水2 0ml/kg ,左肺自主通气的方法模拟淡水淹溺造成直接和间接肺损伤模型。于淹溺后 1、2、4、6、8h五个观察时间点采集肺组织 ,逆转录 聚合酶链 (RT PCR)方法检测肺组织中粘附分子的表达 ,光镜下病检测中性粒细胞 (polymorphonuclearneutrophil,PMN)在肺组织的浸润情况。 结果 淹溺后各时相点肺组织中性粒细胞大量浸润 ,ICAM 1、VCAM 1mRNA表达均有显著的升高。ICAM 1mRNA淹溺后 4h达高峰为 (87 9± 6 1 ) % (P <0 0 1 ) ,6h后开始下降。PTX则可以明显抑制这种变化 (P<0 0 1 )。同样VCAM 1mRNA有微量表达 ,PTX组比淹溺组相应时相点表达减少 (P <0 0 1 )。结论 PTX可以通过降低肺淹溺后的ICAM 1、VCAM 1的表达 ,从而减轻PMN在肺内的聚集、活化 。
Objective To investigate the effectsof pentoxifylline on the expression of ICAM 1 and VCAM 1 duringlung drowning injury in canines. Methods 18 canines were divided randomly into 3 groups :control group (Ⅰ);fresh water drowning group (Ⅱ); pentoxifylline treatment group (Ⅲ). The drowning model was established with insertingCarlen's tube to isolate the lungs.Fresh water(20ml/kg) was poured into right lung with the left lung autoventilated , to simulate the process of drowning, which made the lung direct and indirectl injuried. The expression of ICAM 1 and VCAM 1 mRNA was detected by reverse transcription polymerase chain reaction (RT PCR) before drowning and after drowning for 1,2,4,6,8 hours respectively. The infiltration of PMN was examined by histopathologic methods. Results After drowning the infiltration of PMN and the expression of ICAM 1 and VCAM 1 were significantly increased, and went up to the highest after drowning for 4 hours. But PTX could inhibit the expression in group Ⅲ. Conclusion PTX may reduce the aggregation and activation of PMN in the lung through its inhibition effect to on the expression of ICAM 1,VCAM 1 after pulmonary drowning ,and pretect the lung from pulmonary drowning injury.
出处
《临床麻醉学杂志》
CAS
CSCD
2003年第9期545-549,共5页
Journal of Clinical Anesthesiology
