摘要
目的 探讨全血基因组DNA的快速纯化方法。方法 微量全血通过红细胞裂解后,得到白细胞,再通过白细胞裂解,高盐离子去除蛋白,异丙醇沉淀即可得到高纯完整的全血基因组DNA。结果 该方法简单快速,得到的产品产率高(6.94μg/300μl全血),纯高度(A_(260)/A_(280)=1.82,A_(260)/A_(230)=2.08),完整性好(单一条带)。结论 该方法快速、高效,不仅缩短了实验时间,而且还提高了产品质量,可完全满足实验室快速分析的要求。
Objective To research the rapid purification for whole blood genomic DNA. Methods Highly purificated and integral DNA can be obtained by a process, including red cells lysis and white cells lysis, then protein was removed with high concentration ion and nuclear acid precipitation with iso-propyl alcohol. Results This method is simple and rapid with high yield (6.94μg/300μl whole blood) , high purity (A260/A280 = 1.82 A260/A280 = 2.08) and integrity (single DNA band). Conclusion This is one kind of rapid and efficient purification method. Not only does it shorten the experiment' s time but also improve the quality.The product can satisfy the requirement of the experiment thereafter.
出处
《洛阳医专学报》
2003年第1期7-8,共2页
Journal of Luoyang Medical College
