摘要
目的 对树、熊猴感染人乙型肝炎病毒 (HHBV)后对肝细胞病变进行动态观察。方法 10只成年树 ,2 8只熊猴接种含人乙型肝炎病毒 (HBV)血清后 ,定期肝活检 ,采用HE染色、免疫组化、原位杂交对实验动物肝组织进行研究分析。结果 80 %树感染HHBV后 ,通过免疫组化在肝组织内可找到乙型肝炎病毒表面抗原 (HBsAg) ,5 0 %通过原位杂交可检测到HBVDNA。有 2 5 %熊猴的肝组织内可检测到HBsAg ,但信号较弱 ,而肝组织内未发现HBVDNA。结论 树感染HHBV后 ,肝细胞内出现病理改变 。
Objective To serially observe the pathologic changes in livers of tree shrews and macaca assamensises infected with HHBV Methods 10 adult tree shrews and 28 macaca assamensises were inoculated with HBV rich human sera The liver of the animals were regularly biopsied The liver samples were examined histopathologically by HE staining Some samples were stained for HBsAg by immunohistochemistry (IH), and HBV DNA by in situ hybridization (ISH) Results HBsAg in 80% of tree shrews infected with HHBV can be detected by IH, HBV DNA in 50% of those can be found by ISH. The positive rates of HBsAg in macaca assamensises ' livers were 25% by IH, none HBV DNA was detected Conclusion The tree shrew model seems to be applicable for the research of human hepatitis B
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2003年第3期244-246,T003,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家"九五"科技攻关课题 (96 90 6 3 0 10 )
