摘要
收集了国内 15个省 2 6个不同地区的烟粉虱种群 ,根据有关报道合成了一个随即引物H16 ,对不同地区的烟粉虱DNA进行了RAPD -PCR扩增 ,根据扩增结果分析了不同烟粉虱种群的生物型。结果表明 ,在以上不同省市和地区中 ,北京、山东等 11个省 19个地区都有烟粉虱B型的分布。以烟粉虱B型为防治对象 ,比较了使用高效氯氰菊酯、高效大功臣 2种化学农药 ,和应用桨角蚜小蜂、粉虱座壳孢 2种寄生性天敌 ,对黄瓜上烟粉虱种群的控制效果 ,结果表明在一个世代内 ,喷施高效大功臣 (10 0 0× ,1次 ) ,喷施粉虱座壳孢 (5× 10 6 个孢子 /ml,2次 ) ,和释放桨角蚜小蜂 (3头雌蜂 /株 ,2次 ) ,对烟粉虱种群的控制作用分别达到 78.2 1%、95 .74 %和 5 7.5 8% ,三者都可以对烟粉虱起到较好的防治效果。
Twenty-six populations of Bemisia tabaci (Gennadius) were collected for test from 26 different cities related to 15 provinces in China, and the biotypes of the different whitefly populations were identified by a RAPD PCR method with a primer H16. The results showed that 19 whitefly populations from 11 provinces were B biotype, including Beijing, Shandong, Anhui, Jiangsu, Jiangxi, Fujian, Guizhou, Chongqing, Guangxi, Guangdong and Hainan. Different methods were studied to control whitefly populations on cucumber plants in greenhouses experiments: the parasitoid, Eretmocerus sp. (3 females/plants/once/3 weeks and 3 females/plant/twice/3weeks), the entomopathogenic fungus, Aschersonia aleyrodis (5×106spores/ml/once/3weeks and 5×106spores/ml/twice/3weeks), the chemical insecticides, super-imidacloprid and super-cypermethrin (1000×/once/3weeks). The result showed that the population of B. tabaci B biotype decreased with 40.62%, 57.58%, 56.69%, 95.74%, 78.21% and 43.51%, respectively.
出处
《华东昆虫学报》
2003年第2期27-31,共5页
Entomological Journal of East China
基金
国家"十五"攻关项目 (2 0 0 1BA5 0 9B0 6 0 4 )
广东省自然科学基金项目 (0 10 312 )
华南农大校长基金项目 (4 200-k03130)
关键词
烟粉虱
B生物型
分布
防治措施
Bemisia tabaci , B biotype, distribution, control
