摘要
①目的 探讨不同临床分期胃癌病人外周静脉及脾静脉血中Th1、Th2 细胞因子变化与人体细胞免疫功能的关系。②方法 应用ELISA方法检测 33例胃癌病人、10例良性胃疾病病人 (对照组 )外周静脉及脾静脉血中Th1细胞因子粒细胞 巨噬细胞集落刺激因子 (GM CSF)、Th2 细胞因子白细胞介素 4 (IL 4 )的含量。③结果胃癌病人外周静脉及脾静脉血中GM CSF含量低于对照组 (t=2 .38、2 .4 2 ,P <0 .0 5 ) ,而IL 4含量高于对照组 (t=4 .11、18.94 ,P <0 .0 1) ;胃癌病人脾静脉血中IL 4含量高于外周静脉血 (t=30 .30 ,P <0 .0 1)。各期胃癌病人中 ,Ⅱ、Ⅲ、Ⅳ期病人外周静脉及脾静脉血中GM CSF含量均低于Ⅰ期胃癌病人 ,而IL 4含量则高于Ⅰ期胃癌病人 (F =9.2 9～ 2 7.81,q =4 .5 7～ 30 .2 6 ,P <0 .0 1)。④结论 胃癌病人Th1、Th2 细胞因子的失衡可能是导致胃癌病人免疫偏移的原因之一。
Objective\ To study the changes of plasma levels of T helper 1 (Th 1) and T helper 2 (Th 2) cytokines and investigate causes of immune deviation in patients with gastric cancer (GC) in different clinical stages. \ Methods\ Plasma levels of granulocyte macrophage colony stimulating factor (GM CSF)and interleukin 4(IL 4),in both peripheral vein (PV) and splenic vein (SV), were measured by enzyme linked immunosorbent assay (ELISA) in 33 patients with GC and 10 patients with benign gastric diseases.\ Results\ Plasma levels of GM CSF in PV and SV of GC patients were significantly lower than those in controls ( t= 2.38 ,2.42,P <0.05), while IL 4 were significantly higher in GC patients ( t=4.11,18.94,P <0.01). IL 4 levels were higher in SV than in PV ( t=30.30,P <0.01). Plasma levels of GM CSF in PV and SV of GC patients in Stage Ⅱ, Stage Ⅲ and Stage Ⅳ were significantly lower than that in Stage Ⅰ,whereas, plasma levels of IL 4 in PV and SV of GC patients in Stage Ⅱ, Ⅲand Ⅳ were significantly higher than that in Stage Ⅰ( F=9.29-27.81,q=4.57-30.26,P <0.01). \ Conclusion\ Dysequilibrium of Th 1 and Th 2 might be one of the causes leading to immune deviation in patients with gastric carcinoma.
出处
《青岛大学医学院学报》
CAS
2003年第3期225-227,共3页
Acta Academiae Medicinae Qingdao Universitatis
