摘要
目的 研究电刺激对失神经支配骨骼肌萎缩的影响。 方法 选用 SD大鼠 16只 ,建立双侧下肢失神经支配腓肠肌的实验模型 ,电刺激右侧腓肠肌为实验侧 ,左侧不作电刺激为对照侧 ,术后 2、4周分别观察肌肉的组织学 ,超微结构 ,纤颤电位波幅及 Na+ - K+ - ATP酶和 Ca2 + - ATP酶活性变化。 结果 实验侧术后 2、4周肢体肌细胞直径及截面积较对照侧下降速度明显减慢 ;电刺激能延缓肌细胞的线粒体 ,肌质网退变 ,但对肌肉纤颤电位波幅无明显影响 ;实验侧术后 2、4周 Na+ - K+ - ATP酶活性下降速度比对照侧分别慢 15 .5 9%和 2 7.38% ;实验侧术后 2、4周 Ca2 + -ATP酶活性下降速度较对照侧分别慢 4 .83%和 2 1.6 4 %。 结论 电刺激对失神经支配骨骼肌的组织学、电生理及酶组织化学的各项指标均有保护作用 ,是延缓肌肉萎缩的有效方法。
Objective To study the influence of the electric stimulation on denervated muscle atrophy. Methods Sixteen SD rats were made the model of denervated skeletal muscle in two lower limbs by cutting off the sciatic nerve and femoral nerve. The right gastrocnemius muscle was stimulated with JNR Ⅱ nerve & muscle recovery instrument by skin as the experimental side and the left was not treated as the control side. The muscle histology, ultrastructure, fibrillation potential amplitude, Na + K + ATPase and Ca 2+ ATPase activities were observed 2 weeks and 4 weeks after operation. Results Electric stimulation could protect mitochondria and sarcoplasmic reticulum from the degeneration. The reduction rates of muscle cell diameter and cross section in the experimental side were slower significantly than those in control side. There was no influence on fibrillation potential amplitude in the both sides after electric stimulation .The reduction rates of Na + K + ATPase activity in the experimental side were slower 15 59 % and 27 38 % respectively than those in the control side. The reduction rates of Ca 2+ ATPase activity in the experimental side were slower 4.83 % and 21.64% respectively than those in the control side. Conclusion The electric stimulation can protect muscle histology, electrophysiology and enzymic histochemistry of denervated skeletal muscle from the degeneration. The electric stimulation is an effective method to prevent and treat muscle atrophy.
出处
《中国修复重建外科杂志》
CAS
CSCD
2003年第5期396-399,共4页
Chinese Journal of Reparative and Reconstructive Surgery
