摘要
目的 探讨CDDP诱导Scaber凋亡特点和与凋亡相关蛋白表达的关系。方法 选用CDDP诱导膀胱癌细胞株Scaber凋亡 ,运用HE、TUNEL、透射电镜等方法分别检测细胞凋亡、观察凋亡的形态变化特点及应用S P免疫组化方法检测凋亡相关蛋白bcl 2、Bax、caspase 3表达的动态变化。结果 ①顺铂可诱导Scaber细胞凋亡 ,其诱导凋亡作用在一定范围内具有时间及剂量依赖性 ;②CDDP诱导Scaber细胞凋亡时 ,bcl 2降低、Bax增加 ,同时出现了bcl 2的核周及核内移位 ;③不同浓度的CDDP可使Scaber细胞中caspase 3的表达增强 ,并具有时间及剂量的依赖性。结论 顺铂可诱导Scaber细胞凋亡 ,CDDP诱导Scaber细胞凋亡与bcl 2降低、Bax增加及bcl 2移位有关 ;CDDP诱导Scaber细胞凋亡是Caspase 3依赖性的。
AIM To investigate the effect of cisplatin on apoptosis in Scaber cell. METHODS The apoptotic cells were detected by TUNEL,HE,eletronic micrpscopy. RESULTS Treatment of Scaber cells with CDDP resulted in characteristics typical of apoptosis. CDDP induced apoptosis of Scaber cells in time and concentration dependent manner. To further investigate the mechanism of apoptosis induced by CDDP, the expressions and activity of apoptosis associated proteins such as bcl 2, bax and caspase 3 were examined using S P method.The results showed: CDDP caused time and concentration dependent decreases in bcl 2 and increased in bax proteins.CDDP bcl 2 and its translocation to perinuclei and nuclei. The expression of caspase 3 in Scaber cell were determined during apoptosis induced by CDDP. CONCLUSION Our investigetion showed that the apoptosis induced by CDDP is related to the increase of bax protein, and the decrease of bcl 2 protein. and its translocation to perinuclei and nuclei.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第10期1143-1147,共5页
Chinese Pharmacological Bulletin
基金
安徽省教委自然科学基金资助课题(Nojl10 12)
安徽医科大学博士科研基金资助
