摘要
目的对不同品种大豆(黑豆、青豆、黄豆、褐豆)为原料加工的大豆黄卷和淡豆豉进行含量测定,分析大豆黄卷和淡豆豉的实验室加工品和市售品中异黄酮含量的差异,为大豆黄卷和淡豆豉的制备研究及临床用药提供科学依据。方法以不同品种大豆为原料加工大豆黄卷和淡豆豉,采用紫外分光光度法测定总黄酮含量,使用HPLC法对药材中大豆苷、大豆苷元、染料木素、染料木苷含量进行测定。采用C_(18)色谱柱(250mm×4.6 mm,5μm),以甲醇-1%醋酸溶液为流动相,进行梯度洗脱,检测波长260 nm,柱温30℃,流速1.0 ml·min^(-1)。结果黄豆加工成的大豆黄卷中结合型糖苷大豆苷和染料木苷总量最大,含量范围为0.1090%～0.1431%,黑豆加工成的淡豆豉中游离型苷元大豆苷元和染料木素总量最大,含量范围为0.0937%～0.1628%。结论黄豆适宜加工成大豆黄卷,黑豆适宜加工淡豆豉,这也是市售品大豆黄卷和淡豆豉质量参差的原因。
Objective The study aimed to determine the contents of the Sojae Semen Germinatum and Sojae Semen Praeparatum.To analyze the difference of isoflavone content before and after processing of different varieties of Glycine max,and to provide scientific basis for the technological research and clinical application of Sojae Semen Germinatum and Sojae Semen Praeparatum.Methods UV spectrophotometry was used to determine total flavonoids,the contents of daidzin,daidzein,genistein,and genistin in the medicinal materials were determined by HPLC.Using C18 column(250 mm×4.6 mm,5μm),with a methanol-1%acetic acid solution as the mobile phase,a gradient elution was performed with a detection wavelength of 260 nm,a column temperature of 30℃,and a flow rate of 1.0 ml·min-1.Results The largest total amount of daidzin and genistin in Sojae Semen Germinatum processed with yellow soybean was the highest,content range 0.1090%to 0.1431%.The content of free aglycones in Sojae Semen Praeparatum processed with black soybean was the highest,content range 0.0937%to 0.1628%.Conclusion Yellow soybean are suitable for the processing of Sojae Semen Germinatum,and black soybean are suitable for the processing of Sojae Semen Praeparatum,which is in line with the currently circulating on the market.
作者
刘秀玉
王利丽
杨灏
陈随清
LIU Xiu-yu;WANG Li-li;YANG Hao;CHEN Sui-qing(Henan University of Chinese Medicine,Zhengzhou,Henan 450046,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2019年第2期341-345,共5页
Lishizhen Medicine and Materia Medica Research
基金
中医药公共卫生服务补助专项"全国中药资源普查项目"(财社[2017]66号)
关键词
大豆黄卷
淡豆豉
异黄酮
Sojae Semen Germinatum
Sojae Semen Praeparatum
Iasoflavone
