摘要
目的探讨菌群感应信号分子N-酰基高丝氨酸内酯(N-acyl homoserine lactones,AHLs)对小鼠肠道微生物菌群构成的影响,为感染状态下肠道微生态的紊乱补充理论依据。方法 3-oxo-C_(10)-HSL分子经腹腔注射小鼠后,于24 h分别在小鼠十二指肠及空回肠部位用拭子涂抹法取样,并通过Illumina Hiseq 2500高通量测序平台对样品16S rDNA测序,并进行微生物菌群分类构成及多样性分析。结果在实验小鼠肠道内共检出22个细菌门,109个细菌属;对照组和3-oxo-C_(10)-HSL处理组小鼠肠道不同部位菌群均以厚壁菌门(>50%)和变形菌门(>20%)为主,但两组在属水平菌群构成有明显差异,对照组优势菌属依次为葡萄球菌、埃希氏-志贺菌、乳球菌、假单胞菌、肠球菌,3-oxo-C_(10)-HSL处理组优势菌属主要为肠球菌、埃希氏-志贺菌、葡萄球菌;多样性和丰度分析提示3-oxo-C_(10)-HSL处理组肠道菌群多样性均有提高,大肠埃希菌属等主要优势菌丰度显著上升,而且空回肠部位的菌落丰度最高。结论 AHLs对小鼠肠道菌群构成影响较小,而对菌群内部某些特定类群结构变化影响较明显。
Objective Study the effection of N-acyl homoserine lactones(AHLs)on gut microbes flora in mice so as to supply the theoretical basis of intestinal microecological disorder under infection status.Method After the mice were intraperitoneal injected with 3-oxo-C10-HSL for 24 h,samples of mice duodenum and jejuno-ileum contents were acquired with swab smear method.The sample 16 S rDNA sequencing was detected by Illumina Hiseq 2 500 high-throughput sequencing platform,and the classification of microbial flora composition and diversity were analyzed.Result There were 22 phyla and 109 genera in all identified in the mice gut,and Firmicutes(>50%)and Proteobacteria(>20%)dominated the microbiota.The control group and 3-oxo-C10-HSL treatment group had obvious difference in the level of flora composition,for the former the dominant genera mainly included Staphylococcus,Escherichia-Shigella,galactococcus,Pseudomonas and Enterococcus,and for the latter the dominant genera mainly included Enterococcus,Escherichia-Shigella,and Staphylococcus.It was also revealed that the 3-oxo-C10-HSL treatment could improve the gut microbial diversity,and the abundance of dominant genera such as Escherichia-Shigella and jejuno-ileum microbiota significantly rose.Conclusion AHLs had little effect on gut microbiota in mice,but some specific microbial taxa would be significantly affected.
作者
付凯飞
李军
王欲晓
吴成林
钦可为
周丽君
FU Kaifei;LI Jun;WANG Yuxiao;WU Chenglin;QIN Kewei;ZHOU Lijun((Former name)Navy General Hospital Center for Basic Medical Science,Beijing 100048,China;(Former name)Navy General Hospital Scientific Research Department,Beijing 100048,China)
出处
《实验动物科学》
2019年第2期27-32,共6页
Laboratory Animal Science
基金
军队重点课题(批准号:BHJ14J004)
