摘要
目的:研究食管癌细胞在炎症相关细胞因子作用下miR-302b表达情况,以明确miR-302b的表达水平是否与食管癌肿瘤相关性炎症有关。方法:选择4株食管癌细胞及1株永生化食管上皮细胞株作为对照。用含LPS、IL-6、IFN-γ、TGF-β的RPMI 1640培养基以及普通RPMI 1640培养基培养细胞。于刺激0h、12h、24h、48h提取总RNA,qRT-PCR检测miR-302b表达水平。结果:食管癌细胞miR-302b的表达水平较永生化食管上皮细胞的表达水平低;炎症相关细胞因子可以抑制食管癌细胞miR-302b的表达水平,而且随着刺激时间的延长,对miR-302b的抑制率逐渐升高;而永生化食管上皮细胞miR-302b的表达无明显变化。结论:miR-302b的表达与食管癌肿瘤相关性炎症具有相关性。
Objective: To explore the expression of miR- 302 b in human esophageal cell lines treated with inflammation- associated cytokines and to determine whether the expression of miR- 302 b was related to cancer related inflammation( CRI). Methods: Four esophageal cancer cell lines and one immortalized esophageal epithelial cell line( controls) were selected and cultured respectively with RPMI 1640 medium containing LPS,IL- 6,IFN- γ,TGF- βand normal RPMI 1640 medium. The total RNAs were extracted at 0h,12 h,24h and 48 h after treatment and then the miR- 302 b expression levels were detected with qRT- PCR. Results: The expression level of miR- 302 b in esophageal cancer cell lines was lower than that in immortalized esophageal epithelium cell line. The inflammation- associated cytokines can inhibit the expression of miR- 302 b in a time dependent manner in esophageal cancer cell lines.However,the expression level of miR- 302 b had no significant change in immortalized esophageal epithelial cell line.Conclusion: The expression of miR- 302 b was correlated with CRI in esophageal cancer.
出处
《现代肿瘤医学》
CAS
2016年第5期690-693,共4页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(编号:81302055)
