摘要
为研究膜联蛋白A2(ANXA2)基因与人结直肠癌Caco2细胞凋亡之间的关系,采用RNAi技术沉默Caco2细胞的ANXA2表达后,利用流式细胞仪分析细胞凋亡水平,并利用激光共聚焦显微镜和透射电子显微镜对凋亡细胞进行形态学检测。流式细胞仪检测结果显示,ANXA2表达被抑制后细胞线粒体正常膜电位发生改变(P<0.05),线粒体结构受损;同时,形态学检测结果表明,ANXA2表达被抑制后细胞出现了较为明显的凋亡样特征。结果提示:ANXA2在人结直肠癌Caco2细胞的高表达能一定程度地促进细胞生长,抑制ANXA2表达可以诱导细胞出现一定程度的凋亡样形态特征,但不足以引起细胞广泛而明显的凋亡;推测ANXA2表达水平并不是唯一影响瘤细胞凋亡的因素。该结果支持关于ANXA2具有作为肿瘤靶向诊断、治疗靶分子潜能的推论。
To study the relationship between ANXA2 and the apoptosis of Caco2 cells,the expression of ANXA2 in Caco2cells was inhibited by siRNA.Cell apoptosis was further investigated by flow cytometry(FCM),and morphological characteristics were observed with laser scanning confocal microscopy(CLSM)and transmission electron microscopy(TEM).The results showed that the fluorescence density of the cells was significantly reduced in ANXA2 interfered groups(P<0.05),suggesting that the normal membrane potential of mitochondria has been changed.Moreover,some characteristics of apoptosis cells were also observed in ANXA2 silenced cells compared to control group.Taken together,these results indicate that the excessive expression of ANXA2 in Caco2cells could promote the proliferation of Caco2 cells.Moreover,some morphological characteristics of apoptosis may be induced by silencing ANXA2 in Caco2cells,but it is insufficient to cause widely apoptosis or obvious characteristics of cell apoptosis.We suppose that the excessive expression of ANXA2 is not unique route of anti-apoptosis factors of tumor.These results further confirm that ANXA2 has great potential as a targeting molecule of tumor diagnosis and treatment.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第1期75-79,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
陕西省自然科学基金资助项目(SF2009178)
