摘要
[目的]观察加压输注全血过程中红细胞计数及形态、血钾浓度的变化。[方法]取人去白细胞全血,连接输血器及不同型号静脉留置针(18G、20G、22G、24G),对血袋施以不同的压力,观察未加压及施加100mmHg(1mmHg=0.133kPa)、200mmHg、300mmHg血液流经针头末端后血液中红细胞计数及血钾浓度的变化,并对其中20G样本涂玻片,在油浸镜下观察红细胞形态的变化。[结果]不同压力下血液流经输血器及不同型号静脉留置针后红细胞计数及血钾含量变化差异无统计学意义(P>0.05);普通光镜下显示,随着压力的增加,视野中的粗刺状、椭圆状、梭状细胞数逐渐增加,有些出现细胞碎片。[结论]在压力300 mmHg以内,采用18G、20G、22G、24G静脉留置针输血,短时间内血液中红细胞计数及血钾浓度无明显变化,但有红细胞形态异常。
Objective:To observe RBC count and morphologic changes,serum potassium changes during compression transfusion of WBC-removal whole blood.Methods:Human WBC-removal whole blood was selected, then connecting apparatus transfusion with different numbers types of intravenous detaining needle(18G,20G,22Gand 24G).And different pressures had exerted on blood bags.Then RBC count and serum potassium concentration changes under different pressures(100mmHg,200mmHg, 300mmHg)as blood flew out pinhead end had been observed.Among which,samples of 20Ghad smeared on glass slide and RBC morphologic changes had been observed under oil immersion lens.Results:RBC count and serum potassium changed a little under different pressures as blood flew through apparatus transfusion with different numbers types of intravenous detaining needle.And the results showed under common light microscope that with the pressure increased,coarse-prick,oblonga,and spindle cells counts in visual fields increased gradually.Some cells had occurred splinters.Conclusion:Applying 18G,20G,22Gand 24Gintravenous detaining needle for blood transfusion under less than 300mmHg pressure,no significant RBC count change was found in blood in short time.However, RBC morphologic change had been found.
出处
《护理研究（上旬版）》
2014年第1期27-30,共4页
Chinese Nursing Researsh
基金
山西省高校科技研究开发项目
编号:200233
关键词
加压输血
血液
静脉留置针
压力
红细胞计数
血钾
红细胞形态
compression transfusion
blood
intravenous detaining needle
pressure
RBC count
serum potassium
RBC morphous
