摘要
目的制备载辛伐他汀(simvastatin,SIM)大孔磷酸钙骨水泥(calcium phosphate cement,CPC)支架,并对其理化性质进行评价。方法以十二烷基硫酸钠(sodiumdodecyl sulfate,SDS)作为液相,将不同量SIM粉末加入CPC作为固相,制备载辛伐他汀大孔磷酸钙骨水泥支架。并对其初始凝固时间、力学性能、孔隙率、相转化等理化性质进行评价。结果 SDS对CPC的初始凝固时间无明显影响,但其可显著降低其压缩强度;SDS可促使CPC内部形成均匀分布的大孔,大孔的直径在50~120 m之间,当SDS浓度为300mM时,孔隙率可达26.7%;沉积羟基磷灰石结晶的尺寸随着SDS浓度增加而减小。SIM对CPC初始凝固时间无显著影响;支架内SIM含量达10%时可显著降低CPC的压缩强度;扫描电镜结果显示SIM表面有羟基磷灰石结晶沉积,SIM对大孔形状、结晶尺寸无影响;X射线衍射检测结果表明:理盐水浸润7天后,所有样本的主要成分为羟基磷灰石结晶。结论 SDS对CPC的初始凝固时间、相转化无显著影响;SDS可抑制羟基磷灰石结晶的生长,产生更多、尺寸更小的结晶,这些小的结晶相互缠绕可部分补偿大孔导致的CPC力学性能的下降。少量的SIM对CPC的理化性质无显著干扰,当含量达到10%时,可显著降低CPC的力学性能。
Objective To prepare SIM-containing macroporous CPC and evaluate the physicochemical properties. Methods An aqueous solution of Na2HPO4 with different amount of SDS as used as the liquid phase. Different amount of SIM was homogenized with the solid phase. The cement paste was made by mixing the powder and liquid phases at a powder-to-liquid ratio of 2.5 g/ml. The initial setting time, macroporosity, Compressive strength, transformation of phase of the test samples were investigated. Results SDS had no significant effect on the cement's initial setting time, however could decrease the compressive strength significantly. On the fracture surfaces, there was evidence of spherical macroporous structures(50 to 120 m) homogeneously distributed in the case of CPCs that contained SDS. The macroporosity could reach to 26.7%, when the concentration of SDS was 300mM; The size of crystals was smaller as the reduction of concentration of SDS. SIM had no significant effect on the cement's initial setting time and macroporosity and did not lead to a significant decrease in the cement's compressive strength for SIM content below 10%; The SEM results showed that needle-like OHAp crystals were formed around and on the surface of the SIM rod. The XRD results showed that apatite was the predominant cement phase after seven days of soaking. Conclusion Introduction of SDS led to the incorporation of interconnected macrospores into the CPCs without significantly interfering with initial setting time, transformation of solid phase to hydroxyapatite, and biocompatibility. Regardless of the amount of SIM(1% to 10% wt) added to the CPC, it was compatible with the basic CPC. A large amount of SIM(10% wt) decreased the compressive strength of the cement.
出处
《生物骨科材料与临床研究》
CAS
2014年第4期14-17,20,共5页
Orthopaedic Biomechanics Materials and Clinical Study
关键词
磷酸钙骨水泥
十二烷基硫酸钠
辛伐他汀
药物缓释系统
Calcium phosphate bone cements
Sodium dodecyl sulfate
Simvastatin
Drug delivery systems
