摘要
目的:构建肝脏组织选择性转录因子的调控网络。方法:按照基因功能的差异对组织选择性Affymetrix探针集(共3919条探针)进行聚类,挑选肝组织选择性转录因子(liver-selective transcription factor,LSTF)基因进行研究。收集各基因上游的500个核苷酸启动子序列,再预测这些基因对应的转录因(transcription factors,TFs)以及转录因子结合位点(transcription factor binding sites,TFBS),并构建LSTF的转录调控网络(transcriptional regulatory network)。结果:获得4个LSTF基因,利用两款软件预测得到4个相同TFs,每个LSTF基因上游存在多个TFs的结合位点,根据3款蛋白质相互作用软件和相关文献作出的转录因子调控网络(包含LSTF、预测的TFs和与其密切相关的其它几个基因),显示各个基因受到复杂的调控,其中肝组织选择性转录因子ID2和PROX1处于网络的显著位置。结论:结合文献分析,ID2和PROX1参与调节肝细胞生长分化等过程,在LSTF的转录网络中可能发挥重要的调控功能。
Objective:To construct transcription regulatory network of liver-selective transcription factors(LSTF).Methods;Tissue-selective Affymetrix probes set(3919 probes all together) were classified based on their functional categories.LSTF genes were then selected for further analysis.The 500bp upstream sequences of all LSTF genes were extracted for predicting binding sites of known transcription factors(TFs).Transcription regulatory network was constructed using LSTF and related TFs as input.Results;Four LSTF genes were obtained from functional classification and tissue selectivity screen,which share 4 upstream TFs in common as predicted by two programs.Transcription regulatory network among LSTF,predicted TFs and some other related genes demonstrates a fairly complicated topology,with ID2 and PROX1 centering in the network.Conclusions;Based on existing literatures,ID2 and PROX1 genes may play important role in regulating LSTF genes transcription,because they are involved in hepatocyte growth and differentiation,etc.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第S1期7-10,共4页
China Biotechnology
基金
广东省生物芯片重点实验室基金资助项目(2004B60144)
关键词
组织选择性
肝
转录因子
转录因子结合位点
转录调控网络
Tissue selectivity
Liver
Transcription factors(TFs)
Transcription factor binding sites(TFBS)
Transcription regulatory network
