摘要
以苍山县四六瓣大蒜为原料,采用响应面法对大蒜素的提取工艺进行优化。以腐败希瓦氏菌为受试菌种,大蒜素对腐败希瓦氏的抑菌圈直径(cm)作为判定指标,确定大蒜素提取最适宜的酶解环境和提取溶剂分别为恒温摇床和乙酸乙酯,且大蒜素含量和抑菌圈直径之间线性拟合良好。选取酶解温度(℃)、摇床速度(r/min)和提取时间(min)为单因素,运用响应面Box-Behnken设计3因素3水平试验,并以大蒜素对腐败希瓦氏菌的抑菌圈直径为响应值,建立试验模型,优化工艺参数。结果表明,该二次模型能较好拟合因素与响应值之间的关系。在该模型中,预测最佳提取条件为:酶解温度25℃、摇床转速65 r/min、提取时间70 min。在此条件设置下,抑菌圈直径的实测值为(3.95±0.08)cm,与预测值3.92 cm接近。因此,基于Box-Behnken优化设计的条件准确度较高,可作为优化大蒜素在水产品中保鲜试验的依据。
The response surface method was used to optimize the extraction process of allicin from four and six clove of garlic in Cangshan County.The antibacterial zone diameter(cm)of allicin against Shewanella putrefaciens was used as the determination index.The results showed that the optimal enzymatic hydrolysis environment and the extraction solvent were ethyl acetate in a constant temperature shaker,and the linear fitting between the allicin content and the diameter of the inhibition zone was perfect.The enzymatic hydrolysis temperature(℃)and shaking table speed(r/min)and extraction time(min)were selected as the single factors,and Box-Behnken in the response surface was used to design a three-factor and three-level test.The results showed that the quadratic model could well fit the relationship between factors and response values.In this model,the optimal extraction conditions were predicted:enzymatic hydrolysis temperature 25℃,shaking table rotation speed 65 r/min,extraction time 70 min.Under this condition,the measured value of inhibition zone diameter was(3.95±0.08)cm,which was close to the predicted value of 3.92 cm.Therefore,the condition based on Box-Behnken optimization design was of high accuracy,which could be used as the basis for the preservation experiment of allicin in aquatic products.
作者
许萍
黄敏
廖涛
熊光权
陈玉霞
张金木
王炬光
白婵
XU Ping;HUANG Min;LIAO Tao;XIONG Guangquan;CHEN Yuxia;ZHANG Jinmu;WANG Juguang;BAI Chan(College of Chemistry and Environmental Engineering,Wuhan Institute of Technology,Wuhan430250;Irradiation Preservation Technology Key Laboratory of Sichuan Province,Sichuan Institute of Atomic Energy,Chengdu610101;Institute of Agro-ProductsProcessing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Sciences/Hubei Engineering Research Center for Farm Products Irradiation,Wuhan430064)
出处
《食品科技》
CAS
北大核心
2019年第9期270-276,共7页
Food Science and Technology
基金
四川省原子能研究院辐照保藏四川省重点实验室开放基金项目(FZBC2018002)
现代农业产业技术体系专项资金项目(CARS-46)
湖北省农业科技创新中心项目(2019-620-000-001-036)
