摘要
采用高效液相色谱法(high performance liquid chromatography,HPLC)、液相色谱-质谱法(liquid chromatography-mass spectrometry,LC-MS)鉴定红枣低聚糖成分,建立一种测定多种糖分的HPLC分析方法,测定不同品种红枣低聚糖含量。样品经70%(体积分数)乙醇提取,Ca柱HPLC分离分析,采用阳离子检测模式的LC-MS进行定性分析,峰面积外标法进行定量分析。通过HPLC保留值和LC-MS法鉴定出红枣中含有蔗果三糖和蔗果四糖。建立的HPLC分析方法可使红枣的5种主要糖分实现很好的分离,在0.30 mg/mL^10.0 mg/mL之内线性关系良好,r=0.999 6(n=5),RSD为0.53%~0.83%,加标回收率为97%~108%,对32种红枣的低聚糖进行含量测定,结果显示5种糖分总含量为223 mg/g F.W^395 mg/g F.W,其中金丝新1号含量最高。两种低聚糖含量范围为3.1 mg/g F.W^14.8 mg/g F.W,其中阜平大枣含量最高。蔗果三糖和蔗果四糖是红枣中代表性低聚糖,在成熟期果实中其含量在1%(鲜重)数量级。
High performance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS)for determination of the oligosaccharides in jujube were used. A method based on HPLC was established for simultaneous determination of various saccharides in jujube,to determine the content of oligosaccharides in different kinds of jujube. the samples were extracted by 70 % ethanol,the separation was achieved on a HPLC with Ca-column,the qualitative analysis was achieved on a LC-MS with cationic mode,the external calibration curves were used in quantification. Kestose and nistose trihydrate were identified by the retention values of HPLC and LC-MS. 5 kinds of main saccharides were satisfactorily separated by the established HPLC method,the linear range was 0.30 mg/mL-10.0 mg/mL( r = 0.999 6),the relative standard deviations(RSD)of repeated measurement were between 0.53 %-0.83 %,standard addition recovery rate was between 97 %108 %. By the method,we analyzed the content of jujube oligosaccharides from 32 different varieties,it turned out that the total content of 5 saccharides was between 223 mg/g F.W-395 mg/g F.W,the highest content of the jin si xin NO.1,the total content of 2 oligosaccharides was between 3.1 mg/g F.W-14.8 mg/g F.W,the highest content of the fuping jujube. Kestose and nistose trihydrate were the representative oligosaccharides in jujube,the content reached up to 1 % in the mature period.
出处
《食品研究与开发》
CAS
北大核心
2017年第15期148-151,共4页
Food Research and Development
基金
国家林业公益行业科研专项(201304708)
关键词
红枣
低聚糖
HPLC
含量测定
jujube
oligosaccharides
HPLC
content determination
