摘要
以饲喂高脂膳食的仓鼠为动物模型,研究根皮苷在喂饲高脂膳食仓鼠体内的抗氧化活性。实验动物随机分为对照组和3个实验组(3、6、9 g/kg根皮苷),6周后测定仓鼠血清、心脏、肾脏及肝脏中抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)和总抗氧化力(total antioxidant capacity,T-AOC)的活力、丙二醛(malonaldehyde,MDA)含量及采用荧光定量聚合酶链式反应(real time-polymerase chain reaction,RT-PCR)法检测肝脏中抗氧化相关基因mRNA表达水平。抗氧化酶活力测定结果显示,给予根皮苷后血清、心脏、肾脏及肝脏中SOD、GSH-Px、CAT酶活力均有升高,氧化产物MDA含量显著降低。RT-PCR结果显示,3个不同剂量根皮苷实验组CuZn-SOD、Mn-SOD、GSH-Px、血红素加氧酶1(heme oxygenase 1,HO-1)、核因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)mRNA表达水平与对照组相比均极显著升高(P<0.01);给予6、9 g/kg根皮苷,CAT基因表达水平较对照组极显著升高(P<0.01)。因此,根皮苷对高脂膳食喂饲仓鼠氧化应激损伤的保护作用可能是通过激活抗氧化基因Nrf2表达,并上调抗氧化基因SOD、GSH-Px、CAT、HO-1 mRNA表达水平,进而增加抗氧化酶的活力实现的。
Excessive exposure to dietary lipids causes deranged homeostasis of cellular energy metabolism and oxidative damage. Phlorizin is rich in phenolic hydroxyl groups which have been proved to play an antioxidant role in vivo and in vitro. This study aimed to investigative the antioxidant activity of phlorizin in hamsters fed a high fat diet. Totally, 36 hamsters were fed either control diet or one of the three experimental diets containing 3, 6, or 9 g/kg phlorizin for 6 weeks. Then the enzyme activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT), total antioxidant capacity(T-AOC) and the content of malonaldehyde(MDA) in hamster serum, heart, kidney and liver were assayed. Finally, the mRNA expression levels of antioxidant enzymes including SOD, GSH-Px, CAT, heme oxygenase-1(HO-1) and F-E2-related factor 2(Nrf2) were detected by real-time PCR(RT-PCR). Antioxidant enzyme activity assays showed that the activities of SOD, GSH-Px and CAT in serum, heart, kidney and liver were increased and the content of MDA was significantly reduced by dietary phloridzin supplementation. RT-PCR results showed that the mRNA expression levels of CuZn-SOD, Mn-SOD, GSH-Px, HO-1 and Nrf2 were significantly increased compared with those of the control group(P < 0.01), while CAT gene in 6 and 9 g/kg groups were significantly increased(P < 0.01) compared with the control group. Therefore, the protection of phloridzin against oxidative stress injury in hamsters fed a high fat diet may be mediated through the induction of the expression of Nrf2 gene and the increases in the mRNA expression levels of SOD, GSH-Px, CAT, and HO-1, which in turn increases the activities of antioxidant enzymes.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第15期221-225,共5页
Food Science
基金
国家自然科学基金青年科学基金项目(31201322)
"十二五"国家科技支撑计划项目(2012BAD33B05)
天津市高等学校科技发展基金计划项目(20100609)
关键词
根皮苷
抗氧化
酶活力
基因表达
phlorizin
antioxidant
enzyme activity
gene expression
