摘要
为了更好地开发蛹虫草资源,对蛹虫草菌株富集微量元素硒的培养条件进行了优化,选择最佳参数的低能离子束注入蛹虫草,通过石墨炉原子吸收光谱法检测注入前后菌丝体中硒元素的含量。结果表明:蛹虫草发酵富硒的最优培养条件为蛋白胨质量浓度3 g/100 mL、葡萄糖质量浓度3 g/100 mL、亚硒酸钠质量浓度为8?g/mL、pH值为7,此时,富硒率为21.58%。离子束最佳注入参数为:注入离子为N+,注入能量10 keV,注入剂量1.82×1015 ions/cm2,选育出富集微量元素硒较高的10株菌株,最高富硒率为30.97%,比出发菌株提高了近42%。
In the present study, investigations were carried out to optimize the medium components for enhanced enrichment of the dietary microelement selenium(Se) in Cordyceps militaris mycelia. The Cordyceps militaris strain No.13 preserved in our laboratory was improved by low-energy nitrogen ion beam implantation under optimal operating conditions to increase Se enrichment in the mycelia. The Se contents in the original and mutant strains were determined by graphite furnace atomic absorption spectrometry(GFAAS). Results showed that the optimal medium components for Se enrichment in Cordyceps militaris mycelia were determined as follows: peptone 3 g/100 mL, glucose 3 g/100 mL and Na2SeO3 8 μg/mL at pH 7.0, resulting in a selenium enrichment rate of 21.58%. The optimal parameters for lowenergy nitrogen ion beam implantation were achieved at an energy of 10 keV and a dose of 1.82×1015 ions/cm2. Ten mutant strains showing higher Se-enriching capacity were obtained. The highest Se enrichment rate of 30.97% was observed, nearly 42% higher than that of the original strain.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第15期136-140,共5页
Food Science
基金
国家自然科学基金面上项目(81273004
31270577)
江苏省高校自然科学基金项目(10KJD180006)
关键词
蛹虫草
硒
低能离子束
富集
注入
Cordyceps militaris
selenium
low-energy ion beam
enrichment
implantation
