摘要
为提高烟曲霉WHSW-01产壳聚糖酶的能力,首先确定了该菌产壳聚糖酶受碳源的诱导作用。通过在以壳聚糖为基础碳源的培养基中添加不同碳源,考察该菌细胞生长及产酶的情况,确定了壳聚糖水解液对烟曲霉WHSW-01产壳聚糖酶具有最好的诱导作用。通过考察壳聚糖水解液添加量及添加时间对烟曲霉WHSW-01产壳聚糖酶的影响,探讨了壳聚糖水解液对烟曲霉WHSW-01产壳聚糖酶的最佳诱导条件:发酵12 h后添加发酵液总体积40%的壳聚糖水解液对烟曲霉产壳聚糖酶进行诱导,有利于壳聚糖酶的产生,在此条件下烟曲霉发酵产酶高峰期壳聚糖酶酶活为6.78 U/m L。
In order to improve the production of chitosanase from Aspergillus fumigates WHSW-01,effects of different carbon sources on chitosanase production was studied. Aspergillus fumigatus WHSW-01 chitosanase was considered as an inducible enzyme. Results indicated that chitosan hydrozate was the optimal inducer for the production of chitosanase from Aspergillus fumigatus WHSW-01. The optimal addition amount of chitosan hydrozate was 40% and the optimal addition time of it was after the fermentation continued 12 h. Finally,6. 78 U / m L of chitosanase was obtained when Aspergillus fumigates WHSW-01 was cultured under these conditions.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第5期110-115,共6页
Food and Fermentation Industries
关键词
烟曲霉:壳聚糖酶
诱导
诱导条件
Aspergillus fumigatus
chitosanase
induction
induction conditions
