摘要
目的建立一种快速、低成本检测染色梅鱼中日落黄、柠檬黄的方法。方法将染色梅鱼剥皮,鱼皮经70%甲醇氨水提取液提取,经EclipseXDB-C_(18)色谱柱分离,以甲醇和乙酸铵水溶液为流动相,进行梯度洗脱,流速为1.0 mL/min,柱温35℃,利用紫外检测器检测日落黄、柠檬黄的含量。结果日落黄、柠檬黄在1.25、2.5、12.5、25 mg/kg添加水平的加标回收率为80%～90%,相对标准偏差小于10%(n=6),柠檬黄检出限为0.05 mg/kg,日落黄检出限为0.025 mg/kg。结论该方法快速、准确、成本低,适合染色梅鱼中日落黄、柠檬黄的快速检测。
Objective To develop a method for determination the sunset yellow and lemon yellow in Collichthys niveatus by liquid chromatography.Methods The dyed Collichthys niveatus was peeled,and the fish skin was extracted with 70%methanol ammonia water extract.The samples were separated with a Eclipse XDB-C18 column at the flow rate of 1.0 mL/min by gradient elution using methanol and ammonium acetate in aqueous solution as the mobile phase,the temperature of column was 35℃,and the content of sunset yellow and lemon yellow was detected by ultraviolet detector.Results The standard recoveries of sunset yellow and lemon yellow at 1.25,2.5,12.5,25 mg/kg were 80%~90%,and the relative standard deviations were less than 10%(n=6).The detection limit of lemon yellow was 0.05 mg/kg,and the detection limit of sunset yellow was 0.025 mg/kg.Conclusion This method is rapid,accurate and low cost,which is suitable for the rapid detection of sunset yellow and lemon yellow in the dyed Collichthys niveatus.
作者
严卓彦
吴俣
YAN Zhuo-Yan;WU Yu(The Public Detecion and Inovation Service Center of Putuo District,Zhoushan 316000,China)
出处
《食品安全质量检测学报》
CAS
2019年第9期2786-2790,共5页
Journal of Food Safety and Quality
关键词
日落黄
柠檬黄
梅鱼
液相色谱法
sunset yellow
lemon yellow
Collichthys niveatus
liquid chromatography
