摘要
目的观察TGF-β_1/Smad通路是否参与机械牵张促进大鼠成纤维细胞胶原合成的影响。方法原代培养大鼠成纤维细胞,分成五组培养在细胞力学加载装置中,分别给予如下刺激:未加力组:不加力,培养条件同以下各组;加力1组:力学加载拉伸幅度10%,48 h;加力2组:力学加载拉伸48 h,幅度30%;4加力+抑制剂1组:先用10μmol/L LY2109761处理2 h后加力,力学加载拉伸幅度10%48 h;5加力+抑制剂2组:先用10μmol/L LY2109761处理2 h后加力,力学加载拉伸48 h,幅度30%。处理后采用western blot技术检测各组Ⅰ型和Ⅲ型胶原合成情况。结果与未加力组比较,加力1组和加力2组均有显著的促进成纤维细胞Ⅰ型胶原合成的作用,加力1组有显著的促进成纤维细胞Ⅲ型胶原合成的作用;与加力1组比较,加力+抑制剂1组细胞Ⅰ型和Ⅲ型胶原合成显著减少,与加力2组比较,加力+抑制剂2组细胞Ⅰ型和Ⅲ型胶原合成显著减少。结论周期性牵张刺激能够促进成纤维细胞Ⅰ型和Ⅲ型胶原的合成,TGF-β_1/Smad信号转导通路参与了这种促进作用。
Objective To observe weather TGF- β1/ Smad pathway is involved in the collagen synthesis acceleration caused by mechanical stretching. Methods Primary cultured rats fibroblasts were divided into five groups. 1) None stretch group: rats fibroblasts were cultured without stretching. 2) First stretch group:tensile rate of cultured rats fibroblasts was 10% for 48 hours. 3) Second stretch group: tensile rate of cultured rats fibroblasts was 30% for 48 hours. 4) First stretch group with inhibitor: the fibroblasts were treated by LY2109761 for two hours before stretched to 10% for 48 hours. 5) Second stretch group with inhibitor: the fibroblasts were treated by LY2109761 for two hours before stretched to 30% for 48 hours. After stretched,the five groups of fibroblasts were tested by western blot for the synthesis of collagen- Ⅰ and Ⅲ. Result The collagen- Ⅰ synthesis of first stretch group and second stretch group were significantly higher than none stretch group. The collagen- Ⅲ synthesis of first stretch group was significantly higher than none stretch group. Collagen- Ⅰ and Ⅲ synthesis of first stretch group with inhibitor was significantly lower than the first stretch group. Collagen- Ⅰ and Ⅲ synthesis of second stretch group with inhibitor was significantly lower than the second stretch group. Conclusion Periodic stretching can promote the collagen- Ⅰ and Ⅲ synthesis of fibroblast. TGF- β1/ Smad pathway is involved in the effect.
出处
《世界中西医结合杂志》
2016年第1期34-37,共4页
World Journal of Integrated Traditional and Western Medicine
基金
北京中医药大学2013年青年教师专项研究课题(010060506)
