摘要
目的观察积雪草苷对大鼠心梗后左室功能及左室胶原改建的影响。方法通过结扎雄性SD大鼠左冠状动脉前降支建立心肌梗死模型,随机分为假手术组、心梗模型组和积雪草苷治疗组,积雪草苷治疗组自造模当天灌胃给予积雪草苷连续6 w,其他2组给予等体积生理盐水。各组大鼠分别在术后3 d、1 w、2 w、6 w时点,采用动脉导管测定心功能;氯氨T法测量左室非梗死区心肌羟脯氨酸水平;免疫组化法测定左室非梗死区心肌Ⅰ/Ⅲ型胶原蛋白比值。结果与同时点心梗模型组比较,积雪草苷组大鼠2 w、6 w时LVDP显著降低(P<0.05),+dp/dtmax显著提高(P<0.05),-dp/dtmax显著提高(P<0.01);2 w、6 w时左室非梗死区心肌羟脯氨酸含量显著降低(P<0.01);2 w、6 w时左室非梗死区心肌胶原Ⅰ/Ⅲ比值显著降低(P<0.01)。结论积雪草苷可改善心梗大鼠左室功能,减少左室非梗死区心肌组织胶原异常表达,减轻大鼠心梗后心肌纤维化。
Objective To observe the influence of the asiaticoside on left ventricular function and collagen regeneration of myocardial infarction rats. Methods MI model rats were established by ligation of left anterior descending coronary artery,and randomly divided into three groups: shamoperated group,MI model group and asiaticoside treatment group,then asiaticoside treatment keeps on 6 weeks since the day of operation. At the end of3 d,1 w,2 w and 6 w after the day of operation,the ventricular performance were measured by arterial catheterization; Hydroxyproline of non-infarcted zone in the left ventricle was determined by chloramines T method; Collagen Ⅰ and Ⅲ of non-infarcted zone in left ventriclewere were detected by immunohistochemistry,then calculated the ratio of the Collagen Ⅰ and Ⅲ. Results LVDP of rats in the experimental group significantly reduced in comparison with control group at 2 w and 6 w,+ dp / dtmaxand-dp / dtmaxsignificantly increased,as well as the hydroxyp roline and the ratio of the CollagenⅠ and Ⅲ of non-infarcted zone in left ventricle,nevertheless. Conclusion Asiaticoside can improve left ventricular function after myocardial infarction in rats,and inhibit myocardial fibrosis through decreasing the expression of collagenⅠ and collagenⅢ of left ventricular non-infarcted myocardium.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第8期19-22,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
河南省南阳市2012年科技发展计划(2012GG066)
