摘要
调节性B细胞(regulatory B cell,Breg)是机体内分泌IL-10的主要细胞,在肝癌等恶性肿瘤免疫中发挥重要作用。研究将2015年6月至2017年12月来河北医科大学第四医院就诊的前列腺癌和良性前列腺增生患者纳入研究范围,免疫组化法检测良、恶性组织中IL-10水平和Ki-67增殖指数;流式细胞术检测外周血CD19^+IL-10^+Breg百分比;MTT实验检测前列腺癌细胞增殖率;Transwell侵袭实验检测前列腺癌细胞侵袭能力。免疫组化结果显示良性前列腺增生组织中IL-10平均光密度(average optical density, AOD)为12.60±2.72,Ki-67增殖指数为(3.00±0.84)%,前列腺癌组织中IL-10的AOD为86.10±11.49,Ki-67增殖指数为(45.30±4.11)%(t=6.23,P<0.000 1;t=10.08,P<0.000 1);流式细胞术检测结果显示良性前列腺增生患者外周血中Breg百分比为(0.14±0.03)%,前列腺癌患者外周血中Breg百分比为(0.41±0.04)%(t=5.082,P<0.000 1);相关性分析结果显示前列腺癌患者和良性前列腺增生患者外周血Breg百分比与组织中IL-10表达水平和Ki-67增殖指数均呈显著正相关(r=0.701 9,P=0.000 6;r=0.784 3,P<0.000 1);MTT及Transwell实验结果显示Breg促进前列腺癌细胞增殖和侵袭,IL-10在其中发挥重要作用。综上,Breg在前列腺癌患者外周血中所占比例显著升高,能够通过分泌IL-10促进前列腺癌细胞增殖和侵袭,这可能为前列腺癌的免疫治疗提供新靶点。
Regulatory B cells(Breg) are important immune cells that secrete IL-10 and play an important role in the immunity to malignant tumors such as hepatocellular carcinoma. Patients who visited our hospital during June 2015 to December 2017 forprostate cancer or benign prostatic hyperplasia were included in this study. The expressions of IL-10 and Ki-67 proliferation index were analyzed by immunohistochemistry;Flow cytometry was used to measure the proportion of CD19^+IL-10^+Breg in peripheral blood;MTT test was used to detect the proliferation of prostate cancer cells;Transwell invasion test was used to test the invasion ability of prostate cancer cells. The results of immunohistochemistry showed that the average optical density of IL-10 was 12.60±2.72 and the proliferation index of Ki-67 was(3.00±0.84)% in benign prostatic hyperplasia, the average optical density of IL-10 was 86.10±11.49 and Ki-67 proliferation index was(45.30±4.11)% in prostate cancer tissue(t=6.23, P < 0.000 1;t=10.08, P < 0.000 1). The results of flow cytometry showed that the proportion of Breg in peripheral blood of patients with benign prostatic hyperplasia was(0.14±0.03)%, and that in the patients with prostate cancer was(0.41±0.04)%(t=5.082, P < 0.000 1). Correlation analysis showed that the proportion of Breg cells in peripheral blood of both patients with prostate cancer and benign prostatic hyperplasia had significant positive correlation with IL-10 expression and Ki-67 proliferation index(r=0.701 9, P=0.000 6;r=0.784 3, P < 0.000 1). MTT and Transwell experiments showed that Breg promoted proliferation and invasion of prostate cancer cells, and IL-10 played an important role in these processes. In conclusion, the proportion of Breg in the peripheral blood of prostate cancer patients is significantly higher, and Breg promotes the proliferation and invasion of prostate cancer cells through the secretion of IL-10. Our study provides a new target for the immunotherapy of prostate cancer.
作者
马永良
樊博
任宗涛
张晓宇
刘彬
卫书飞
MA Yong-liang;FAN Bo;REN Zong-tao;ZHANG Xiao-yu;LIU Bin;WEI Shu-fei(Department of Urology,Fourth Hospital of Hebei Medical University,Shijiazhuang050000,China)
出处
《现代免疫学》
CAS
CSCD
北大核心
2019年第1期8-11,63,共5页
Current Immunology
