摘要
目的探讨肥胖相关(FTO)基因rs9939609单核苷酸多态性对低出生体重儿及肥胖的影响。方法随机收集2012年12月至2015年12月在浙江省绍兴市妇幼保健院产科出生的新生儿375例,分为低出生体重组(175例)与健康对照组(200例),采用PCR直接测序法对外周血白细胞进行FTO基因rs9939609单核苷酸多态性分析,随访至24月龄并记录婴幼儿的身高、体重及计算体质量指数(BMI)等数据,研究基因型与体重、身高及BMI的关系。结果①低出生体重组和对照组人群中等位基因分布符合Hardy-Weinberg遗传平衡定律(P值分别为0.29、0.14),说明样本具有群体代表性。FTO基因rs9939609在低出生体重组和对照组中基因频率分别为:AA型0.034和0.020,AT型0.240和0.165,TT型0.726和0.815,两组基因型频率分布差异在共显性模型中无统计学意义(P>0.05),但在显性模型中差异具有统计学意义(χ~2=4.254,P<0.05),相对TT基因型而言,AA+AT能增加低出生体重发病的风险(OR=1.722,95%CI:1.054～2.811,P<0.05);而两组间等位基因频数差异具有统计学意义(χ~2=4.525,P<0.05),相对于T位点而言,A位点是低出生体重的危险等位位点(OR=1.597,95%CI:1.035～2.466,P<0.05)。②随访至24月龄,携带AA+AT基因型婴幼儿体重与TT基因型比较差异均无统计学意义(均P>0.05)。从身高变化来看,携带AA+AT基因型幼儿9月龄、12月龄、18月龄及24月龄的身高均明显低于TT基因型(t值分别为3.50、4.20、8.90、7.50,均P<0.05);从BMI变化来看,携带AA+AT基因型幼儿9月龄、12月龄、18月龄及24月龄的BMI均明显高于TT基因型(t值分别为3.90、4.50、3.10、4.80,均P<0.05)。结论本研究结果显示FTO基因rs9939609单核苷酸多态性与低出生体重发病风险相关,并与婴幼儿时期(9月龄后)身高及BMI的改变有关。
Objective To explore the impact of the FTO gene rs9939609 single nucleotide polymorphism on the incidence of low birth weight and obesity. Methods Totally 375 infants born in the Obstetrics Department of Shaoxing Women and Children’s Hospital of Zhejiang Province from December 2012 to December 2015 were randomly collected and divided into low birth weight group(175 cases) and healthy control group(200 cases). The blood leukocytes of peripheral blood were analyzed for FTO gene rs9939609 single polymorphism by polymerase chain reaction(PCR) combining direction sequencing. Height and weight were measured for body mass index(BMI) from birth to 24 months. Correlation of FTO genotype and infant’s weight, height as well as BMI was analyzed. Results The genotype distributions of the low birth weight group and control group conformed to Hardy-Weinberg equilibrium(HWE)(P value was 0.29 and 0.14, respectively), indicating that the samples were representative. The frequencies of AA, AT and TT of rs9939609 were 0.034, 0.240 and 0.726 in the low birth weight group and 0.020, 0.165 and 0.815 in the control group. The distribution of genotype frequency showed no significant difference in additive model(P>0.05) between two groups while there was significant difference(χ~2=4.254, P<0.05) in dominant model. The odds ratio(OR) of AA+AT was 1.722(95% CI:1.054-2.811, P<0.05), showing significantly increase of the risk of developing low weight at birth in comparison with TT genotype. The difference in allele frequency between two groups was significant(χ~2=4.525, P<0.05). A allele might be the risk allele of low weight at birth in comparison with T allele(OR=1.597, 95%CI:1.035-2.466, P<0.05). After follow-up for 24 months, there was no difference in weight between infants with genotypes of AA+AT and those with TT(all P>0.05). The height of infants with AA+AT genotype was significant lower than that with TT at 9 months, 12 months, 18 months and 24 months(t value was 3.50, 4.20, 8.90 and 7.50, respectively, all P<0.05), but the BMI
作者
吴满武
蒋叶均
杜和春
傅奇琴
WU Manwu;JIANG Yejun;DU Hechun;FU Qiqin(Shaoxing Women and Children’s Hospital,Zhejiang Shaoxing 312000,China)
出处
《中国妇幼健康研究》
2019年第2期148-153,共6页
Chinese Journal of Woman and Child Health Research
基金
2016年绍兴市卫生计生科技计划资助项目[2016cx011]
