摘要
目的 探讨大鼠胃黏膜细胞EGFR活化AP -1的信号传导通路。方法 用EGFR配体TGF -α刺激分离的大鼠胃黏膜细胞 ,Westernblot和EMSA方法检测ERK -1/ 2信号传导通路的活化程度。结果 1nmol/LTGF -α刺激胃黏膜细胞 3 0min不仅显著诱导AP -1的活性 ,而且明显激活MEK和ERK -1/ 2 ;EGFR特异性抑制剂PD15 3 0 3 5或MEK特异性抑制剂PD980 5 9能完全阻断TGF -α诱导的胃黏膜细胞AP -1活化。结论 大鼠胃黏膜细胞EGFR通过ERK -1/ 2信号传导通路激活AP -1。
Objective To explore the signaling pathway of AP-1 activated by epidermal growth foctor receptor(EGFR) in the rat gastric mucosal cells. Methods TGF-α stimulated freshly isolated rat gastric mucosal cells,and Western blot and EMSA detected the activation of ERK-1/2 signaling pathway. Results The exposure of the mucosal cells to 1 nmol/L TGF-α for 30 min significantly induced transcriptional activity of AP-1. This induction was associated with a concomitant activation of MEK and ERK-1/2. TGF-α-induced activation of AP-1 in gastric mucosal cells could be totally abrogated by either PD153035, a specific inhibitor of EGFR tyrosine kinase, or PD98059, a specific inhibitor of MEK. Conclusions Our findings suggest that EGFR AP-1 activates by EPK-1/2 signaling pathway in the rat gastric mucosal cells.
出处
《中国医师杂志》
CAS
2003年第9期1170-1172,共3页
Journal of Chinese Physician
基金
教育部"跨世纪优秀人才培养计划"基金 [(2 0 0 2 ) 4 8]
